ID   RPOB_LACSA              Reviewed;        1060 AA.
AC   Q56P13; Q1KXP3; Q332Z1;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 2.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; ORFNames=PSC012;
OS   Lactuca sativa (Garden lettuce).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC   Lactucinae; Lactuca.
OX   NCBI_TaxID=4236;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15917497; DOI=10.1093/molbev/msi174;
RA   Kim K.-J., Choi K.-S., Jansen R.K.;
RT   "Two chloroplast DNA inversions originated simultaneously during the early
RT   evolution of the sunflower family (Asteraceae).";
RL   Mol. Biol. Evol. 22:1783-1792(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Cisco;
RX   PubMed=16604461; DOI=10.1007/s11248-005-3997-2;
RA   Kanamoto H., Yamashita A., Asao H., Okumura S., Takase H., Hattori M.,
RA   Yokota A., Tomizawa K.;
RT   "Efficient and stable transformation of Lactuca sativa L. cv. Cisco
RT   (lettuce) plastids.";
RL   Transgenic Res. 15:205-217(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Salinas;
RA   Timme R.E., Kuehl J.V., Boore J.L., Jansen R.K.;
RT   "A comparison of the first two published chloroplast genomes in Asteraceae:
RT   Lactuca and Helianthus.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AY865171; AAX58142.1; -; Genomic_DNA.
DR   EMBL; AP007232; BAE47581.1; -; Genomic_DNA.
DR   EMBL; DQ383816; ABD47220.1; -; Genomic_DNA.
DR   RefSeq; YP_398316.1; NC_007578.1.
DR   AlphaFoldDB; Q56P13; -.
DR   SMR; Q56P13; -.
DR   EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_1X118620_mrna; cds-PLY79777.1; gene-LSAT_1X118620.
DR   GeneID; 3772845; -.
DR   Gramene; rna-gnl|WGS:NBSK|LSAT_1X118620_mrna; cds-PLY79777.1; gene-LSAT_1X118620.
DR   KEGG; lsv:3772845; -.
DR   OrthoDB; 944344at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1060
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048027"
FT   CONFLICT        255
FT                   /note="R -> RPAAQAWAAGR (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1060 AA;  120546 MW;  A6F8C2A0D738B485 CRC64;
     MSTIPGFNQI QFEGFCRFID QGLTEELSKF PKIEDTNQEI DFELFLERYQ LVEPSIKERD
     AVYESLTYSS ELYVSARLIW KNDRRRYIQE QTILIGKIPL MTSLGAFIVN GIYRIVINQI
     LQSPGIYYQS ELNDNGISVY TGTIISDWGG RLELEIDRKT RIWVRVSRQQ KLSILVLLSA
     MGLNIREILE NVCYPELFLS FLNDKKQIGS KENAILEFYQ QFACVEGDPV FSESLSKDLQ
     KKFFQQRCEL GGIGRRNMNR RLNLDIPQNN TFLLPRDILA AADRLIRIKF GMGTLDDMNH
     LQNKRIRSVA DLLQEQFGLA LVRLENMARG NIYAALKHNW TPTPQNLVNS TPLTDTYKVF
     FRLHPLSQVL DRTNPLTQIV HGRKLSYLGP GGLTARTATF PIRDIHPSHY GRICPIDTSE
     GINVGLIGSL AIHARIGRWG SLESPFYKIS ERSKGARMLY LSPGRDEYYM VAAGNSLALN
     QGIQEEQVVP ARYRQEFLTI AWEQVHLRSI FSFQYFSIGA SLIPFIEHND ANRALMSSNM
     QRQAVPLSQS EKCIVGTGLE GQAALDSGAL AIAEHEGEII YTDTDKILLS GNGDTLRIPL
     VMYQRSNKNT CMHQKPQVQR GKCIKKGQIL AYGAATVGGE LALGKNVLVA YMPWEGYNFE
     DAVLISERLV YEDIYTSFHI RKYEIQINQG SERVTNEIPH LEVHLLRNLD KNGIVMLGSW
     VETGDILVGK LTPQMVKESS YAPEDRLLRT ILGMRVYTSK ETCLKLPIGG RGRVIDVRWV
     QSSKTDETEK TESIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP YLQDGRPVDM
     VFNPLGVPSR MNVGQIFESS LGLAGGLLDR HYRIAPFDER YEQEASRKLV FSELYEASKQ
     TVNPWIFEPE SPGKSRIFDG RTGDPFEQPV IIGKPYILKL IHQVDDKIHG RSSGRYSRLT
     QQPLKGRAKK GGQRVGEMEV WALEGFGVAY ILQEMLTYKS DHIRARQEVL GTIIFGGRIP
     TPEDAPESFR LFVRELRSLA LELNHFLVSE KTFQLNRKEA