RPOB_LEPBP
ID RPOB_LEPBP Reviewed; 1228 AA.
AC B0SSI4;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LEPBI_I1972;
OS Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris).
OC Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX NCBI_TaxID=456481;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Patoc 1 / ATCC 23582 / Paris;
RX PubMed=18270594; DOI=10.1371/journal.pone.0001607;
RA Picardeau M., Bulach D.M., Bouchier C., Zuerner R.L., Zidane N.,
RA Wilson P.J., Creno S., Kuczek E.S., Bommezzadri S., Davis J.C., McGrath A.,
RA Johnson M.J., Boursaux-Eude C., Seemann T., Rouy Z., Coppel R.L.,
RA Rood J.I., Lajus A., Davies J.K., Medigue C., Adler B.;
RT "Genome sequence of the saprophyte Leptospira biflexa provides insights
RT into the evolution of Leptospira and the pathogenesis of leptospirosis.";
RL PLoS ONE 3:E1607-E1607(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000786; ABZ98074.1; -; Genomic_DNA.
DR RefSeq; WP_012388945.1; NC_010602.1.
DR AlphaFoldDB; B0SSI4; -.
DR SMR; B0SSI4; -.
DR STRING; 456481.LEPBI_I1972; -.
DR KEGG; lbi:LEPBI_I1972; -.
DR HOGENOM; CLU_000524_4_1_12; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR BioCyc; LBIF456481:LEPBI_RS09740-MON; -.
DR Proteomes; UP000001847; Chromosome I.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1228
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141706"
SQ SEQUENCE 1228 AA; 137913 MW; 564A6AE40D8B66EE CRC64;
MHTRMQIRNR VNFGKITDLN LLPNLIYVQK KSFDWFLQSE VKDPTKRLNQ GLEAVFRESF
PIESPNNDMV MEYGHYVLGE PKRDPQECKD TDSSFAVPLK AVIRLIIKDT GEIREQVVYM
GDLPVMTDHG TFIINGAERV VVSQLHRSPG IFFSYDQVRD TFSARVIPYR GSWLEFEMDN
KGILVAKIDR KKKFPATLLV KAMGMGTNEE VLRLFYGSSK MKIAGANPKD LKRLIGRRTI
ADIINMETGE VMLDAGSKIN EDNISILREM KVKEVDVIEF PKGKDNPVLI NCLEKDGVND
YEDAVKKFHT IMRPGEPSTI ENAEAELKRL FFSPKTFDLG IVGRYKINSK FEFNNPKEFS
KADDRVLRKQ DIIETVRYLV MLMSEAENYY PDDIDHLGNR RIRSVGELIA NQLKLGFSRV
ERVIKERMTV QEPEQQTPQL LISIKPITAV INEFFGSSQL SQFMDQTNPL AELTHKRRLN
ALGPGGLSRD RAGFEVRDVH YSHYGRMCPI ETPEGPNIGL ILSMSSFARV NDYGFIETPY
RLVKNGKVQK QVEYLTADKE EYHYMAQSNS TVDEKGEFTS KLISTRHRGD FPFRSPAEIQ
YMDLAPLQVV SVSTALIPFL EHDDANRALM GSNMQRQAVP LLTEEAPFVG TGMEARAAYD
AGVCIVAKKD GVVSKVDATG VWIKEDQSKE IVHYPLIKFK KTNQGTCFNQ KPNVSMLHTT
TGGKVSKVSK ERVEVTTPNG EKETHELLLS DEVQFHAVVK EGQEVGIGAP VAGQIIKGEK
YGDFGQILQK GTVLANGPST DAGYLALGRN VLVAFMPWEG YNFEDAILIS ERIIKDDVFS
SIHIEEFEIQ ARETKLGQEQ ITRDIPNLSD KAFRDLDESG VIRVGAEVKP GDILVGMVTP
KGETDLTPEY KLLHSIFGEK AKEVRDSSLR MPNGFEGTVI DIKRYSRETG DELAAGVEEM
VKVYVARKRK LLVGDKMAGR HGNKGVVARV MAQEDMPYME DGSPVDIVLN PLGVPSRMNL
GQIFETQLGF AAKKLGINFE TPVFDGASEG DVNDFCKKAG LPENSKFQLY DGRTGEKFIN
QVFCGYIYML KLAHLVDDKI HARSTGPYSL VTQQPLGGKA QFGGQRLGEM EVWALEAYGA
SHTLQELLTI KSDDMLGRAR IYEAIVKGIH SIKPGIPESF NVLVQELRGL ALDIIIKDSE
GLEVDISDYE DEFSKNKKKI KFETIENV
