ID   RPOB_LEUCK              Reviewed;        1204 AA.
AC   B1MVW8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LCK_01548;
OS   Leuconostoc citreum (strain KM20).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=349519;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KM20;
RX   PubMed=18281406; DOI=10.1128/jb.01862-07;
RA   Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S.,
RA   Lee S., Park H.-S., Park Y.-H., Oh T.K.;
RT   "Complete genome sequence of Leuconostoc citreum KM20.";
RL   J. Bacteriol. 190:3093-3094(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; DQ489736; ACA83372.1; -; Genomic_DNA.
DR   RefSeq; WP_004904425.1; NC_010471.1.
DR   AlphaFoldDB; B1MVW8; -.
DR   SMR; B1MVW8; -.
DR   STRING; 349519.LCK_01548; -.
DR   EnsemblBacteria; ACA83372; ACA83372; LCK_01548.
DR   GeneID; 61101420; -.
DR   KEGG; lci:LCK_01548; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000002166; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1204
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000141707"
SQ   SEQUENCE   1204 AA;  134455 MW;  E8B957F2C9DE9DD8 CRC64;
     MAGHLVKYGK HRTRRSYASI KEVLDVPNLI EIQTASYQWF LDEGIKEMFG DIMPIDDFQG
     TLSLEYVDYQ LMEPKYNIDE AREHDANYSA PLHVTLRLTN HETGEIKSQD VFFGEFPLMT
     EQGTFIINGA ERVIVSQLVR SPGIYYNQEN DKNGRPRYGT TVIPNRGAWL EYETDAKGIA
     NVRIDRTRKL LMTELVRALG FGSDSDIIDI FSDQYDALNM TLEKDVHKDM SDSRVEEALK
     DVYERLRPGE PKTADSSRAL LVARFFDPKR YDLASVGRYK INKKLSLKTR LLNQTLAETL
     ADPDSGEVIA EKGTLVDKTV MSKLSPFLDR EDFKTTTYTP SGDAVLEEPV TLQKIKIESP
     ENPEKTLLLI GNGHIDENDR TVRPADILAG MNYFLNLQEG IGNVDDIDHL GNRRIRSVGE
     LLQNQFRIGL TRMERVVRER MSIQDANTVT PQQLINIRPV VAAVKEFFGS SQLSQFMDQT
     NPLGEMNHKR RLSALGPGGL TRDRAGVEVR DVHYTHYGRI DPIETPEGPN IGLINSLATY
     GRINKYGFIE TPYRRVDWTT HMVTDKIDYL TADEEDNYIV AQANSPLNDD GSFVHDTVMA
     RFGEENIETP IDRIDYMDVS PKQVVSVGTA AIPFLENDDS NRALMGANMQ RQAVPLLDPH
     APLVGTGIEY KAAHDSGVAM ISRAAGVVEY VDGRQIRVRR EDGQLDTYEL MKFRRSNGGK
     NYNQKPIVHE GEHVEADEVL ADGPSMEKGE LALGQNPLIA FMTWQGYNFE DAIVLNERLV
     REDVYTSIHI EEYDSEARDT KLGPEEMTRE IPNTGEDQLK DLDADGIIRV GAEVHDGDIL
     VGKVTPKGVT ELSAEERLLH AIFGEKAREV RDTSLRVPHG GGGVVQNVRI YTPENGDELA
     PGVNMMVRVY IAQKRKIQVG DKMAGRHGNK GTVSVVVPEE DMPYMPDGTP IDILLSPMGV
     PSRMNIGQVL ELHLGFAAKK LGIHVASPVF DGASDDEIEA ALREAGLPQD GKSVVYDGRT
     GEAFDKRVGV GVMHYMKLAH MVDDKIHARS IGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA
     LEAYGAAYTL QEILTYKSDD VAGRVKVYES IIKGEPIPRP GVPESFRVLV KELQALGLDM
     QVLDNAGDEV ELRQMDEDDS ILPVDALEKL VRSNPDLLNK EDEEVAAAFS KVESRDNTEV
     TDEN