ID   RPOB_LEUMM              Reviewed;        1202 AA.
AC   Q03V60;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LEUM_1825;
OS   Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / DSM
OS   20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523 / NBRC 100496 / NCIMB
OS   8023 / NCTC 12954 / NRRL B-1118 / 37Y).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=203120;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000414; ABJ62912.1; -; Genomic_DNA.
DR   RefSeq; WP_011680408.1; NC_008531.1.
DR   AlphaFoldDB; Q03V60; -.
DR   SMR; Q03V60; -.
DR   STRING; 203120.LEUM_1825; -.
DR   EnsemblBacteria; ABJ62912; ABJ62912; LEUM_1825.
DR   GeneID; 61177760; -.
DR   KEGG; lme:LEUM_1825; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000362; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1202
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300340"
SQ   SEQUENCE   1202 AA;  134288 MW;  782A3095D9D61D09 CRC64;
     MVGHLVKYGK HRTRRSYASI KEVLDVPNLI EIQTASYQWF LNDGIKEMFG DIMPIDDFQG
     TLSLEYVDYQ LMEPKYNIDE AREHDANYSA PLHVTLRLTN HETGEIKSQD VFFGDFPLMT
     EQGTFIINGA ERVIVSQLVR SPGIYYNQTT DKNGRPHFGT TVIPNRGAWL EYETDAKGIA
     NVRIDRTRKL LMTELVRALG FGSDSDIIDI FSDQYDALNM TLEKDVHKDM SDSRVEEALK
     DVYERLRPGE PKTADSSRAL LVARFFDPKR YDLASVGRYK INKKLSLKTR LLNQTLAETL
     ADPDSGEIIA EKGTLVDKEV ISKLTPYLDR EDFKTTTYTP SGDAVLEEPV TLQKIKIESP
     ENPEKTLLLI GNGHIDEDDR TVRPADILAG MNYFLNLQEG VGHVDDIDHL GNRRIRSVGE
     LLQNQFRIGL TRMERVVRER MSIQDANTVT PQQLINIRPV VAAVKEFFGS SQLSQFMDQT
     NPLGEMNHKR RLSALGPGGL TRDRAGVEVR DVHYTHYGRI DPIETPEGPN IGLINSLATY
     GRINKYGFVE TPYRRVDWTT HKVTDKIDYL TADEEDNYIV AQANSPLNED GSFVHNTVMA
     RFGEENIETP IDRIDYMDVS PKQVVSVGTA AIPFLENDDS NRALMGANMQ RQAVPLLDPH
     SPLVGTGIEY KAAHDSGVAM IARASGEVEY VDGRQIRVRR EDGQLDTYEL MKFRRSNGGK
     NYNQKPIVHV GEHIEADEVL ADGPSMEQGE LALGQNPLIA FMTWQGYNFE DAIVLNERLV
     REDVYTSIHI EEYDSEARDT KLGPEEMTRE IPNTGEDQLK DLDADGIIRV GAEVHDGDIL
     VGKVTPKGVT ELSAEERLLH AIFGEKAREV RDTSLRVPHG GGGVVQNVRI YTPENGDELA
     PGVNMMVRVY IAQKRKIQVG DKMAGRHGNK GTVSVVVPEE DMPYMPDGTP IDILLSPMGV
     PSRMNIGQVL ELHLGFAAKK LGIHVASPVF DGASDDEIEM ALREAGLPQD GKSVVYDGRT
     GEAFDKRVGV GVMHYMKLAH MVDDKIHARS IGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA
     LEAYGAAYTL QEILTYKSDD VAGRVKVYES IIKGEPIPRP GVPESFRVLV KELQALGLDM
     QVLDGAGDEV ELRQMDEDDS ILPVDALEKL ARTNPDLLNK DDEEVAAAFS KVEEQSQTFE
     EK