ID   RPOB_LEVBA              Reviewed;        1203 AA.
AC   Q03PU9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LVIS_1698;
OS   Levilactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM
OS   1170 / LMG 11437 / NCIMB 947 / NCTC 947) (Lactobacillus brevis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Levilactobacillus.
OX   NCBI_TaxID=387344;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB
RC   947 / NCTC 947;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000416; ABJ64773.1; -; Genomic_DNA.
DR   RefSeq; WP_011668507.1; NC_008497.1.
DR   AlphaFoldDB; Q03PU9; -.
DR   SMR; Q03PU9; -.
DR   STRING; 387344.LVIS_1698; -.
DR   EnsemblBacteria; ABJ64773; ABJ64773; LVIS_1698.
DR   GeneID; 66421584; -.
DR   KEGG; lbr:LVIS_1698; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001652; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 3.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1203
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300329"
FT   REGION          1167..1203
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1203 AA;  134300 MW;  1B8C3059FE668D1F CRC64;
     MAGHLVKYGK HRTRRSYSRI KEVLDLPNLI EIQTNSYNWF LDEGLRDMFD DIMPIEDFQG
     NLSLEFVDYQ LLEPKYSVDE AREHDANYSA PLHVTLRLTN HETGEIKSQD VFFGDFPLMT
     AQGTFIINGA ERVIVSQLVR SPGVYFNEDT DKNGRTVYGA TVIPNRGAWL EFETDAKNVS
     YVRIDRTRKI PMTELVRALG FGSDDEILDI LGDNESLMQT LEKDIHKNTD DSRVEESLKD
     IYERLRPGEP KTADSSRSLL TARFFDPKRY DMAPVGRYKT NKKLSLKTRL LGLTLAETLA
     DPDTGEIIAA KGTVVDKDVM KSLAPFLDQD DFKMVTFQPS DEAVVTEPLK LQIIKVQSPD
     DPEQEVPVIG NGNIDIKLKH IRPADIIASM NYFFDLQLGI GNTDDIDHLG NRRIRSVGEL
     LQNQFRIGLS RMERVVRERM SIQDAATVTP QQLINIRPVV ASIKEFFGSS QLSQFMDQTN
     PLGELTHKRR LSALGPGGLT RDRAGYEVRD VHYTHYGRMC PIETPEGPNI GLINSLSSYA
     RVNKYGFIET PYRRVSWDTH KVTDKIDYLT ADEEDNYVVA QANTPLNDDG SFNTDTIMAR
     AQSENIETSA DRIDYMDVSP KQVVAVATAC IPFLENDDSN RALMGANMQR QAVPLLNPHA
     PLIGTGIEYK AAHDSGVALI SQHDGTVEYV DAREIRVRRE DGALDTYKLM KFRRSNGGKN
     YNQRPIVKVG DHVDNDEVLA DGPSMENGEL ALGQNPLVAF MTWQGYNFED AIGINERLVR
     DDVYTSIHIE EYESEARDTK LGPEEMTREI PNVGEDALKN LDEDGIIRVG AEVQDGDILV
     GKVTPKGVTE LSAEERLLHA IFGEKAREVR DTSLKVPHGG GGIIQDVKIF TRENGDELSP
     GVNMMVRVYI AQKRKIQVGD KMSGRHGNKG TVSVVIPEED MPYMPDGTPI DIMLSPMGVP
     SRMNIGQVLE LHLGMAARKL GVHMATPVFD GAQDTDIADA VKEAGMAADA KTVLYDGRTG
     EPFDKRVAVG VMHYLKLAHM VDDKMHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL
     EAYGAAYTLQ EILTYKSDDV VGRVKTYEAI VKGDPIPKPG VPESFRVLVK ELQSLGLDMK
     VLNGDKEEIE LRDMDDEDDD VVNVDALSKY AQQQEEQRKA AAQTDESKTA PATKNESQPN
     TQD