ID   RPOB_LISMO              Reviewed;        1184 AA.
AC   Q9RLT9;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=lmo0258;
OS   Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=169963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS RIFAMPICIN RESISTANT.
RC   STRAIN=ATCC 35152 / LMG 10470 / NCTC 7973;
RX   PubMed=10449475; DOI=10.1128/jcm.37.9.2913-2919.1999;
RA   Morse R., O'Hanlon K., Virji M., Collins M.D.;
RT   "Isolation of rifampin-resistant mutants of Listeria monocytogenes and
RT   their characterization by rpoB gene sequencing, temperature sensitivity for
RT   growth, and interaction with an epithelial cell line.";
RL   J. Clin. Microbiol. 37:2913-2919(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35152 / LMG 10470 / NCTC 7973;
RX   PubMed=12361249; DOI=10.1099/00207713-52-5-1477;
RA   Morse R., O'Hanlon K., Collins M.D.;
RT   "Phylogenetic, amino acid content and indel analyses of the beta subunit of
RT   DNA-dependent RNA polymerase of Gram-positive and Gram-negative bacteria.";
RL   Int. J. Syst. Evol. Microbiol. 52:1477-1484(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA   Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA   Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA   Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA   Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA   Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA   Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA   Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA   Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA   Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; Y16468; CAB56706.1; -; Genomic_DNA.
DR   EMBL; AL591974; CAD00785.1; -; Genomic_DNA.
DR   PIR; AC1107; AC1107.
DR   RefSeq; NP_463789.1; NC_003210.1.
DR   RefSeq; WP_003723045.1; NZ_CP023861.1.
DR   AlphaFoldDB; Q9RLT9; -.
DR   SMR; Q9RLT9; -.
DR   STRING; 169963.lmo0258; -.
DR   PaxDb; Q9RLT9; -.
DR   EnsemblBacteria; CAD00785; CAD00785; CAD00785.
DR   GeneID; 987342; -.
DR   KEGG; lmo:lmo0258; -.
DR   PATRIC; fig|169963.11.peg.266; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   PhylomeDB; Q9RLT9; -.
DR   BioCyc; LMON169963:LMO0258-MON; -.
DR   BRENDA; 2.7.9.6; 3045.
DR   Proteomes; UP000000817; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1184
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047917"
FT   REGION          1160..1184
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         473
FT                   /note="D -> N (rifampicin resistant)"
FT   VARIANT         479
FT                   /note="G -> D (rifampicin resistant)"
FT   VARIANT         483
FT                   /note="H -> L (rifampicin resistant)"
FT   VARIANT         483
FT                   /note="H -> Y (rifampicin resistant)"
FT   VARIANT         528
FT                   /note="L -> F (rifampicin resistant)"
FT   VARIANT         530
FT                   /note="N -> Y (rifampicin resistant)"
SQ   SEQUENCE   1184 AA;  132604 MW;  58B4427F44A7290F CRC64;
     MSGHSGHDVK YGRHRTRRSF ARISEVLELP NLIEIQTASY QWFLDEGLRE MFRDISPIED
     FAGNLSLEFI DYDLGEPKYS VEESKNRDAN YAAPLRVKLR LINKETGEVK DQEVFMGDFP
     LMTEMGTFII NGAERVIVSQ LVRSPGVYFN GKLDKNGKKG FGSTVIPNRG AWLEYETDAK
     DVVHVRIDRT RKLPVTVLLR ALGFGSDQEI IDLIGDNDYL RNTLEKDNTD NAEKALLEIY
     ERLRPGEPPT VDNARSLLVS RFFDPKRYDL ASVGRYKINK KLHLKNRLFN QTLAETLVDP
     ETGEIIASKG DILDRRNLDQ IIPNLENGVG FRTLRPTDGV MEDSVLVQSI KIYAPNDEEK
     EINIIGNAYI EENVKHITPS DIISSISYFF NLLHGVGDTD DIDHLGNRRL RSVGELLQNQ
     FRIGLSRMER VVRERMSIQD MTTITPQQLI NIRPVVASIK EFFGSSQLSQ FMDQTNPLGE
     LTHKRRLSAL GPGGLTRERA GYEVRDVHYS HYGRMCPIET PEGPNIGLIN SLSSFAKVNK
     FGFIETPYRR VDPETNRVTD KIDYLTADEE DNYVVAQANS KLDEQGTFTE EEVMARFRSE
     NLAVEKERID YMDVSPKQVV SVATACIPFL ENDDSNRALM GANMQRQAVP LMHPEAPFVG
     TGMEHVSAKD SGAAVTAKHD GIVEHVEARE IWVRRVSLVD GKEVTGGIDK YTLRKFVRSN
     QGTCYNQRPN VAEGDRVVKG EILGNGPSMD SGELALGRNV LVAFMTWDGY NYEDAIIMSE
     RLVKDDVYTS IHIEEFESEA RDTKLGPEEM TRDIPNVGED ALRDLDERGI IRVGAEVKDN
     DLLVGKVTPK GVTELTAEER LLHAIFGEKA REVRDTSLRV PHGGGGIVLD VKIFTREAGD
     ELPPGVNQLV RVYIVQKRKI HEGDKMAGRH GNKGVISRIL PEEDMPFMPD GTPVDIMLNP
     LGVPSRMNIG QVLELHLGMA ARALGIHVAT PVFDGANEED VWSTVEEAGM ARDAKTILYD
     GRSGEAFDNR ISVGVMYMIK LAHMVDDKLH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME
     VWALEAYGAA YTLQEILTIK SDDVVGRVKT YEAIVKGESV PEPGVPESFK VLIKELQSLG
     MDVKMLSADE EEIEMRDMDD DDFTNQNDAF NIVQPENAAA EKTE