ID   RPOB_LOLPR              Reviewed;        1076 AA.
AC   A8Y9G0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LopeCp023;
OS   Lolium perenne (Perennial ryegrass).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Poodae; Poeae; Poeae Chloroplast Group 2 (Poeae type); Loliinae;
OC   Lolium.
OX   NCBI_TaxID=4522;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Cashel;
RX   PubMed=18665252; DOI=10.1371/journal.pone.0002813;
RA   Diekmann K., Hodkinson T.R., Fricke E., Barth S.;
RT   "An optimized chloroplast DNA extraction protocol for grasses (Poaceae)
RT   proves suitable for whole plastid genome sequencing and SNP detection.";
RL   PLoS ONE 3:E2813-E2813(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AM777385; CAO85966.1; -; Genomic_DNA.
DR   RefSeq; YP_001531273.1; NC_009950.1.
DR   AlphaFoldDB; A8Y9G0; -.
DR   SMR; A8Y9G0; -.
DR   GeneID; 5696586; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1076
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329202"
SQ   SEQUENCE   1076 AA;  121441 MW;  075BACE4CF5FB7C9 CRC64;
     MLRNGNEGMS TIPGFSQIQF EGFCRFINQA LAEELNKFPA IKDPDHEIAF QLFAKGYQLL
     EPSIKERDAV YESLTYSSEL YVSARLIFGF DVQKQTISIG NIPIMNSLGT FIINGIYRIV
     INQILLSPGI YYRSELDHKG ISIYTGTIIS DWGGRSELAI DKKERIWARV SRKQKISILV
     LSSAMGSNLR EILDNVSYPE IFLSFPNAKE KKRIESKEKA ILEFYQQFAC VGGDLVFSES
     LCEELQKKFF QQKCELGRIG RRNMNRRLNL DIPQNNTFLL PRDVLAATDH LIGMKFGTGI
     LDDDDMNHLK NKRIRSVADL LQDQFGLALG RLQHAVQKTI RRVFIRQSKP TPQTLVTPTS
     TSILLITTYE TFFGTYPLSQ VFDQTNPLTQ TVHGRKVSCL GPGGLTGRTS SFRSRDIHPS
     HYGRICPIDT SEGINVGLTG SLAIHARINH LWGSIESPFY EISAEKAKKK KSRQVVYLSP
     NRDEYYMIAA GNSLSLNQGI QEEQVVPARY RQEFLTIAWE QIHVRSIFPF QYFSIGGSLI
     PFIEHNDANR ALMSSNMQRQ AVPLSRSEKC IVGTGLERQT ALDSGVSVIA EREGKILSTD
     SHKILLSSSG KTLSIPLVNH RRSNKNNCMH QKSRVPRGKS IKKGQILAEG AATVGGELAL
     GKNVLVAYMP WEGYNFEDAV LISERLVYED IYTSFHIRKY EIQTDTTSQG SAEKITKEIP
     HLEAHLLRNL DRNGVVRLGS WVETSDILVG KLTPQIASES SYIAEAGLLR AIFGLEVSTS
     KETSLKLPIG GRGRVIDVKW IQRDPLDIMV RVYILQKREI KVGDKVAGRH GNKGIISKIL
     PRQDMPYLQD GTPVDMVFNP LGVPSRMNVG QIFESSLGLA GDLLKKHYRI APFDERYEQE
     ASRKLVFSEL YEASKETKNP WVFEPEYPGK SRIFDGRTGD PFEQPVLIGK SYILKLIHQV
     DEKIHGRSTG PYSLVTQQPV RGRAKQGGQR VGEMEVWALE GFGVAHILQE ILTYKSDHLI
     ARQEILNATI WGKRIPNHED PPESFRVLVR ELRSLALELN HFLVSEKNFQ VTREEV