ID   RPOB_LYSSC              Reviewed;        1191 AA.
AC   B1HMZ6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Bsph_4630;
OS   Lysinibacillus sphaericus (strain C3-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX   NCBI_TaxID=444177;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C3-41;
RX   PubMed=18296527; DOI=10.1128/jb.01652-07;
RA   Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M.,
RA   Berry C., Yuan Z.;
RT   "Complete genome sequence of the mosquitocidal bacterium Bacillus
RT   sphaericus C3-41 and comparison with those of closely related Bacillus
RT   species.";
RL   J. Bacteriol. 190:2892-2902(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000817; ACA42074.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1HMZ6; -.
DR   SMR; B1HMZ6; -.
DR   PRIDE; B1HMZ6; -.
DR   EnsemblBacteria; ACA42074; ACA42074; Bsph_4630.
DR   KEGG; lsp:Bsph_4630; -.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000002164; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1191
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000141708"
FT   REGION          1164..1191
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1191 AA;  133879 MW;  DC807F566F7C6CB9 CRC64;
     MNELTGQLVQ YGQHRQRRSF ARIKEVLELP NLIEIQTASY EWFLEEGLRE MFRDISPIED
     FTGNLSLEFI DYSLGDPKYD VDECKERDVT YAAPLRVKVR LYNKETDEVK EQDVFMGDFP
     LMTETGTFII NGAERVIVSQ LVRSPSVYFH DKTDKNGKKG FGATVIPNRG AWLEYETDAK
     DVVYVRIDRT RKLPVTVLLR ALGFGSDQEI IDIIGDNEYL RNTLEKDNSE STEKALLEIY
     ERLRPGEPPT VESAKSLLYS RFFDAKRYDL ANVGRYKMNK KLHIKNRLFN QTIAETLVDP
     ETGEILVEKG TVLDRRTLDK ILPYLEDSSK GIGFRTLSQV GGVLEDDVTI QSIKIYAPKD
     EAQKEINIIS NAYIDEEVKN ITPADVLSSV SYFFNLLYQV GATDDIDHLG NRRLRSVGEL
     LQNQFRIGLS RMERVVRERM SINDTAAIVP QQLINIRPVI ASIKEFFGSS QLSQFMDQTN
     PLAELTHKRR LSALGPGGLT RERAGFEVRD VHYSHYGRMC PIETPEGPNI GLINSLSSFA
     KVNKFGFIET PYRRIDHETG QVTDQIDYLT ADEEDNYYVA QANSPLNPDG SFANDEVVGR
     FRGDNTVFNK AQMDYMDVSP KQVVSAATAC IPFLENDDSN RALMGANMQR QAVPLLNPEA
     PFVGTGMEHV DARDSGAAVV AKYDGIVEHV EARSIHVRRI EVVDGKEVKG DLTKYKLQKF
     IRSNQGTSYN QRPLVKVGER VKPRDILADG PSMEKGELAL GRNVLVAFMT WNGFNYEDAV
     IMSERLVKDD VYTSVHIEEY ESESRDTKLG PEEITRDIPN VGEDALRNLD ERGIIRIGAE
     VRDGDILVGK VTPKGVTELT AEERLLHAIF GEKAREVRDT SLRVPHGAGG IILDVKVFNR
     EDGDELPPGV NQLVRAYIVQ KRKIRVGDKM AGRHGNKGVI SRILPEEDMP FMPDGTPVDI
     MLNPLGVPSR MNIGQVLELH LGMASRYLGV HMATPVFDGA NEEDVWETME EAGMNRDGKT
     ILYDGRSGEP FDNRVSVGIM YMIKLAHMVD DKLHARSTGP YSLVTQQPLG GKAQFGGQRF
     GEMEVWALEA YGAAYTLQEI LTVKSDDVVG RVKTYEAIVK GESVPEPGVP ESFKVLIKEL
     QSLGMDVKML TVNDEEVELR DLDEEEDLQP ADALNIAPQP DTEEEPVESF E