RPOB_MARPO
ID RPOB_MARPO Reviewed; 1065 AA.
AC P06272;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Marchantia polymorpha (Liverwort) (Marchantia aquatica).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Marchantiophyta;
OC Marchantiopsida; Marchantiidae; Marchantiales; Marchantiaceae; Marchantia.
OX NCBI_TaxID=3197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX DOI=10.1038/322572a0;
RA Ohyama K., Fukuzawa H., Kohchi T., Shirai H., Sano T., Sano S., Umesono K.,
RA Shiki Y., Takeuchi M., Chang Z., Aota S., Inokuchi H., Ozeki H.;
RT "Chloroplast gene organization deduced from complete sequence of liverwort
RT Marchantia polymorpha chloroplast DNA.";
RL Nature 322:572-574(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2974085; DOI=10.1016/0022-2836(88)90002-2;
RA Umesono K., Inokuchi H., Shiki Y., Takeuchi M., Chang Z., Fukuzawa H.,
RA Kohchi T., Shirai H., Ohyama K., Ozeki H.;
RT "Structure and organization of Marchantia polymorpha chloroplast genome.
RT II. Gene organization of the large single copy region from rps'12 to
RT atpB.";
RL J. Mol. Biol. 203:299-331(1988).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; X04465; CAA28061.1; -; Genomic_DNA.
DR PIR; A00691; RNLVB.
DR RefSeq; NP_039275.1; NC_001319.1.
DR AlphaFoldDB; P06272; -.
DR SMR; P06272; -.
DR GeneID; 2702533; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IEA:EnsemblPlants.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0035196; P:miRNA processing; IEA:EnsemblPlants.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1065
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048030"
SQ SEQUENCE 1065 AA; 120447 MW; 0BB0AF013A9D3FC3 CRC64;
MEIFILPEFG KIQFEGFNRF INQGLSEELS NFPIIEDIDQ EFEFQIFGEQ YKLAEPLLKE
RDAVYQSITY SSDVYVPAQL TQKKKGKIQK QIVFLGSIPL MNSQGTFVVN GVARVIINQI
LRSPGIYYNS ELDHNGIPIY TGTLISNWGG RLKLEIDGKT RIWARISKKR KVSILVLLLA
MGLNLQNILD SVCYPKIFLE FIKKNTKKEY PNSTEDAIVE LYKHLYCIGG DLFFSESIRK
ELQKKFFQQR CELGKIGRLN LNKKLNLNVP ENEIFVLPQD ILAAVDYLIK LKFGIGTIDD
IDHLKNRRVC SVADLLQDQL KLALNRLENS VLFFFRGATK RKRLPTPKSL VTSTPLIMTF
KEFFGSHPLS QFLDQTNPLT EIVHKRRLSS LGPGGLTRRT ASFQVRDIHA SHYGRICPIE
TSEGMNAGLI ASLAIHAKIS ILGCLESPFY KISKLSNLEE IINLSAAEDE YYRIATGNCL
ALDQNSQEEQ ITPARYRQDF VAIAWEQVHL RSIFPLQYFS VGASLIPFLE HNDANRALMG
SNMQRQAVPL LKPEKCIVGT GIESQTALDS GSVTVSSHGG KIEYLDGNQI ILSLKKKKID
KNLIIYQRSN NSTCMHQKPK VEKQKYIKKG QILADGAATA NGELALGKNI LVAYMPWEGY
NFEDAILINE RLIYEDIYTS IHIERYEIEA RVTSQGPEKF TNEIPHLDDY LLRHLDQNGI
VLTGSWVETG DVLVGKLTPQ ETEENLRAPE GKLLQAIFGI QVATSKETCL KVPPGGRGRV
IDIRLISQED NSANTAQIIH IYILQKRKIQ IGDKVAGRHG NKGIISKILP RQDMPFLQDG
TPIDMILSPL GVPSRMNVGQ IFECLLGLAG SFLHKNYRII PFDERYEREA SRKLVFSELY
KASKKTTNPW LFEPDNPGKN RLIDGRTGEI FEQPITIGKA YMLKLIHQVD DKIHARSSGP
YALVTQQPLR GRSRRGGQRV GEMEVWALEG FGVAYILQEM LTIKSDHIRA RYEVLGAIVT
GEPIPKPNTA PESFKLLVRE LRSLALEINH VIICEKNLKL KLKEI
