ID   RPOB_METCA              Reviewed;        1358 AA.
AC   Q60A06;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=MCA1066;
OS   Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylococcus.
OX   NCBI_TaxID=243233;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA   Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA   Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA   Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA   Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA   Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA   Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA   Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT   "Genomic insights into methanotrophy: the complete genome sequence of
RT   Methylococcus capsulatus (Bath).";
RL   PLoS Biol. 2:1616-1628(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE017282; AAU92668.1; -; Genomic_DNA.
DR   RefSeq; WP_010960366.1; NC_002977.6.
DR   AlphaFoldDB; Q60A06; -.
DR   SMR; Q60A06; -.
DR   STRING; 243233.MCA1066; -.
DR   PRIDE; Q60A06; -.
DR   EnsemblBacteria; AAU92668; AAU92668; MCA1066.
DR   KEGG; mca:MCA1066; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000006821; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1358
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224073"
SQ   SEQUENCE   1358 AA;  151759 MW;  60D325523F2DA705 CRC64;
     MAYSFTEKKR IRKSFGKRQD VLEVPYLLAT QVDSYRRFLQ LDKQPAARSD EGLHAALKSV
     FPIKSHSGNI VLEYVSYRLG DPVFDVKECQ QRGTTYAAPL RVLVRLVVYD KDAPVGAKVV
     RDIKEQEIYM GEIPLMTDNG TFVINGTERV IVSQLHRSPG VFFDHDRGKT HSSGKLLFNA
     RIIPYRGSWL DFEFDHKDCV YVRIDRRRKL PATVLLRALG YDNEQIIAEF FDTNRFLLSS
     AGIQLELIPE RLRGDIASFD IRLGDQIVVE KDHRITARHI RMLQKENVNL LDVPKDYLYG
     KILAHNVVDT STGELIAKVN QEITEDVYAR LVAAAIPEIR TLYVNDLDRG PYISNTMRID
     LTETQLDALV EIYRMMRPGE PPTKEAAQTL FENLFFSAER YDLSAVGRMK FNRRLGRTDP
     TGPGVLENDD IIAVLKELIN IRNGGGTVDD IDHLGNRRVR SVGEMVENQF RLGLVRVERA
     VKERLSLPDA DGLMPQEIIN AKPVAASIKE FFGSSQLSQF MDQNNPLSEV THKRRVSALG
     PGGLARERAG FEVRDVHTTH YGRVCPIETP EGPNIGLINS LAVYSRTNEY GFLETPYRKV
     IDGRVTDQIE YLSAIEEGQY YIAQASASVD ENGMLKDELV SCRHKDEFTL ASRENINYMD
     VSSKQIVSVA ASLIPFLEHD DANRALMGSN MQRQAVPTLR TEKPLVGTGM ERIVARDSGV
     AVVAKRGGTV EFVDASRIVV RVNDEETEAG VPGVDIYNLT KYTRSNQNTC INQRPLVKPG
     DVVARNDVLA DGPSTDMGEL ALGQNLLVAF MPWNGYNFED SILISERVVQ DDRFTTIHIE
     EKTCVARDTK LGPEEITADI PNVGEAALSK LDESGIVYIG AEVKAGDILV GKVTPKGETQ
     LTPEEKLLRA IFGEKASDVK DTSLRVPSGM DGTVIDVQVF TRDGVKKDER ARQIEEAEIE
     RVRKDLNDQL RIIEKDFYQR AEQMVLGKVA DMGPGGLKRG ATITREYLDS IKPAQWLEIR
     LQDEDVNLQI EAIAEQIAQQ REEIAKRLEE KRRKITMGDD LAPGVLKMVK VYLAVKRRIQ
     PGDKMAGRHG NKGVISRIVP VEDMPYSADG TPVDIVLNPL GVPSRMNVGQ VLETHLGWAA
     KGVGLKIGRM LEAKAKIEEI RSFLTQVYNV SGRQEDIASL SDAEVLELAG NLQDGVPMAT
     PVFDGATEED IKAMLRLADL PESGQATLFD GRTGDVFDRP VTVGYMYMLK LNHLVDDKMH
     ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVNGRTKM
     YKNIVDGDHR MEAAMPESFN VLIKEIRSLG INIELEQD