RPOB_METEP
ID RPOB_METEP Reviewed; 1376 AA.
AC A9W8N8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mext_4017;
OS Methylorubrum extorquens (strain PA1) (Methylobacterium extorquens).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=419610;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PA1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C.,
RA Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Marx C., Richardson P.;
RT "Complete sequence of Methylobacterium extorquens PA1.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000908; ABY32387.1; -; Genomic_DNA.
DR RefSeq; WP_003597540.1; NC_010172.1.
DR AlphaFoldDB; A9W8N8; -.
DR SMR; A9W8N8; -.
DR STRING; 419610.Mext_4017; -.
DR EnsemblBacteria; ABY32387; ABY32387; Mext_4017.
DR KEGG; mex:Mext_4017; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR BioCyc; MEXT419610:MEXT_RS20175-MON; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1376
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141709"
SQ SEQUENCE 1376 AA; 153042 MW; 8CF723D7862E8BE7 CRC64;
MANTLVGRKR IRKFFGKIRE VAEMPNLIEV QKASYDQFLM VDEPEGGRAD EGLQSVFKSV
FPISDFASTA LLEFVRYTFE QPKYDVDECR QRGITFAAPL KVTLRLIVFD VDPDTGAKSV
KDIKEQDVYM GDMPLMTDNG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAA
RIIPYRGSWL DVEFDAKDIV HVRIDRKRKL PVTSLLFALG LDGEEILSTF YNRVAYQRDG
ADWRVPFDAE RLKGFKASVD LIDADSGEVV LEAGKKLNAR NARLIGEKGT KFLRAADEDL
IGQYIAEDLV NMKTGEIWAE AGDEISEKLL KSLDDVGVTE LPVLDIDHVN VGPYIRNTLA
VDKNSAREGA LFDIYRVMRP GEPPTLDTAE AMFHSLFFDS ERYDLSAVGR VKMNMRLDLD
AADTVRTLRR EDMLAVVKAL VDLRDGKGEI DDIDHLGNRR VRSVGELMEN QYRLGLLRME
RAIKERMSSV DIDTVMPQDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EVTHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATFARVN KYGFIETPFR
RVKDGVVTDE VAYLSAMEEA KYYVAQANAG MDEGRKLTDD LVVCRRAGEV IVVAPDRVDL
MDVSPKQLVS VAAALIPFLE NDDANRALMG SNMQRQAVPL VRADAPFVGT GMEAVVARDS
GAAIAARRSG IVDQVDATRI VIRASEETDP TKPGVDIYRL QKFQRSNQST CITQKPLVRV
GEPVKKGEII ADGPSTEFGE LALGRNVLVA FMPWNGYNFE DSILLSERIV KDDVFTSIHI
EEFEVMARDT KLGPEEITRD IPNVSEEALK NLDEAGIVYI GAEVHAGDIL VGKITPKGES
PMTPEEKLLR AIFGEKASDV RDTSLRVPPG VTGTIVEVRV FNRHGVDKDE RAQAIEREEI
ERLAKDRDDE QTILDRNTYA RLAEVLIGQS PIAGPKGFRK DTTLTREIIS EYPRSQWWQF
AVVDDRMMTE IEAMQKQYDE SKKRLEQRFL DKVEKLQRGD ELPPGVMKMV KVFVAVKRKI
QPGDKMAGRH GNKGVVSRIV PIEDMPFLED GTHADIVLNP LGVPSRMNVG QILETHLGWA
AAGLGRKVSK AVDAYLKNQD IAPLRAEMEA IYSPSELEGL SDEALAEAGN NVRRGVPMAT
PVFNGAKEAD IETMLEMAGL DRSAQSTLYD GRTGEPFDRK VTMGYIYMLK LHHLVDDKIH
ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVAGRTKV
YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVELTSSKQ QQAANDQIEP PADAAE
