RPOB_METFK
ID RPOB_METFK Reviewed; 1390 AA.
AC Q1H4P4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mfla_0272;
OS Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Methylophilaceae; Methylobacillus.
OX NCBI_TaxID=265072;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KT / ATCC 51484 / DSM 6875;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT "Complete sequence of Methylobacillus flagellatus KT.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000284; ABE48543.1; -; Genomic_DNA.
DR RefSeq; WP_011478640.1; NC_007947.1.
DR AlphaFoldDB; Q1H4P4; -.
DR SMR; Q1H4P4; -.
DR STRING; 265072.Mfla_0272; -.
DR PRIDE; Q1H4P4; -.
DR EnsemblBacteria; ABE48543; ABE48543; Mfla_0272.
DR KEGG; mfa:Mfla_0272; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002440; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1390
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300347"
SQ SEQUENCE 1390 AA; 155365 MW; 85EC16433CE31ECD CRC64;
MSYSFTEKKR LRKSFAKRES VQEVPYLLAM QLESYAAFLQ ADTPADKRTD TGLQAAFSSV
FPIVSHSGNA RLDFVSYNLG QEPFDVKECQ QRGLTYAAPL RVKVRLTIMD KEASKPTVKE
VKEQEVYMGE LPLMTENGSF VINGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDYLYF RVDRRRKMPV TILLKALGFT PEQILETFYD FDTFHIAKKG
VQFELVPERL RGEVAKFDIL DKDGKVIVAK DKRITVKHIR DMEKSGISKI TVPEEFLLGR
SLAAGIVDKT TGEIIANAND EVTESLLDKL READVNKINT LYANDLDHGD YISQTLRIDE
IPDEYSARVA IYRMMRPGEP PTEEAVQALF EGLFFNEDRY DLSAVGRMKF NRRAFPEKIE
ERTAAWLRRF YEKVGPQGAE GPGTLSNDDI LAVIAVLIEL RNGRGEIDDI DHLGNRRIRS
VGELAENQFR AGLVRVERAV KERLSQAESD NLMPHDLINA KPVSSAIREF FGSSQPSQFM
DQTNPLSEIT HKRRVSALGP GGLTRERAGF EVRDVHSTHY GRVCPIETPE GPNIGLINSL
ALYSRTNQYG FLETPYRRVA DNKVSDQIDY LSAIEESQYM IAQANSELDQ DGRFKEDLVS
ARYHNEFTMA QPDRVQYMDV APGQIVSVAA SLIPFLEHDD ANRALMGANM QRQAVPCLRA
EKALVGTGIE RTVAVDSGTV VTARRGGVVD YVDSSRIVVR VHDEEAAAGE VGVDIYNLIK
YTRSNQNTNI NQRPLVNVGD VLARGDVIAD GASTDMGELA LGQNMLVAFM PWNGYNFEDS
ILISERVVAD DRYTSIHIEE LSVVARDTKL GAEEITRDIS NLSERMLGRL DESGIIYIGA
EVEAGDVLVG KVTPKGETQL TPEEKLLRAI FGEKASDVKD TSLRVPSGMS GTVIDVQVFT
REGIERDARA QQIIDDQLAD YKQDLADQMR IVEDDAFGRI RRLIEGKVAT GGPQKLKKGE
TVTAEYLDGL ARYDWFDIRL SDEDVARQLE QLRDSLSQAR IEFDSKFEEK KRKLTQGDEL
PPGVQKMVKV YLAVKRRIQP GDKMAGRHGN KGVVSKIVPI EDMPHMADGT PMDIVLNPLG
VPSRMNIGQI LEVHLGWAAK GLGIRIGNML QAQAKAAELR DFLEQVYNKS NGKSEDIASL
SDDEVIDLAK NLRSGVPFAT PVFDGATEAD IKAMLDLAFP DDDPRTQQLQ FASGKTQVTL
FDGRTGDAFE RPVTVGYMHV LKLHHLVDDK MHARSTGPYS LVTQQPLGGK AQFGGQRFGE
MEVWALEAYG ASYTLQEMLT VKSDDVTGRT KVYENIVKGE HKIDAGMPES FNVLVKEIRS
LAIDIDLDRN
