RPOB_METI4
ID RPOB_METI4 Reviewed; 1289 AA.
AC B3E163;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Minf_0804;
OS Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain
OS V4)).
OC Bacteria; Verrucomicrobia; Methylacidiphilae; Methylacidiphilales;
OC Methylacidiphilaceae; Methylacidiphilum.
OX NCBI_TaxID=481448;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate V4;
RX PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V.,
RA Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.,
RA Feng L., Wang L., Alam M.;
RT "Complete genome sequence of the extremely acidophilic methanotroph isolate
RT V4, Methylacidiphilum infernorum, a representative of the bacterial phylum
RT Verrucomicrobia.";
RL Biol. Direct 3:26-26(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000975; ACD82859.1; -; Genomic_DNA.
DR RefSeq; WP_012463141.1; NC_010794.1.
DR AlphaFoldDB; B3E163; -.
DR SMR; B3E163; -.
DR STRING; 481448.Minf_0804; -.
DR PRIDE; B3E163; -.
DR EnsemblBacteria; ACD82859; ACD82859; Minf_0804.
DR KEGG; min:Minf_0804; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_0; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000009149; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 3.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1289
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141710"
SQ SEQUENCE 1289 AA; 144000 MW; 66C46D997A201C63 CRC64;
MNEKIQRINF GKIREGLSLP NLIEHQTKSY ADFLQLSVAP QERKAEGLHG VFLEVFPIES
YDGKVKLEYV NYEIGESKFS PIDCLRDGKT YAAPLYVTFR LKDEEGVKEE KVYMGELPMM
TPQGSFIING AERVIVNQLH RSPGICFESS FHSNGKTLYS FRIIPDRGSW LEVAFDTNDL
LYVYLDRRKK RRKFLITTLL RALAAALSGP SSPLGSGGDE EIIRLFYTVE ELNLSEGIDE
DKVATKVLVS EVKDPHNPDV VLARAYEPVT RSVVRQLLDV GIQSISVVDV QYDDTLIKCL
KKDPTKDPVE ALKEIYRRLR PGDPPTESNA KLLLKRLLLD PKRYDLGRVG RYKLNQKLNI
QVDPDIRILT WEDLVAATRY LIKLRKGEGI TDDIDHLGSR RVRTVGELVA NQCRMGLART
ERLVKERMTL FDVNTEGMTP QKLINPKALS ATIRDFFARS QLSQLMDQIN PLSELTHKRR
LSALGPGGLS RERAGFEVRD VHPSHYGRIC PIETPEGPNI GLIATMASYS RFNDYGILET
PYRKVLNGKV TNEIVYFTAD QEENYVIAMA NTAVSEDGTI LDQRVAVRFR REFMEVEREK
VEYMDVSPKQ IVSVAAGLIP FLEHDDANRA LMGSNMQRQA VPLIQPEAPI VGTGIEERVA
RDIQAVIVSE VDGTVSSVTG KEIIVTPTGS PVEQKKKKSK QGSEREGIVY KLNKFMRSNA
GTCINQKPIV KKGQVVKKGD VLADGPSTQN GELALGRNLL VAFMPWNGYN FEDAIIVSER
VVKEDLFTSI YIDEFEIVAR DTKLGPEEIT RDIPNVGDEA LKNLGPDGII RVGAEVKPGD
ILVGKITPKS ETELAPEERL LRAIFGEKAA DVKDSSLRVP SGTYGIVMDV RVSSGTARVR
KEKINASEAK QKIKEIEERY DQKEEELREE LTQALSNILL NEKIPLDVVN VETGEIIIPA
NRKITKVLLR KMAQAYDKIE IDPSPIRSKI FEIIGNFEAK FEQLRNDREL ELDQIESGED
TEPGIIKQVK VFIANKRKLS VGDKMAGRHG NKGVVSKIVP VEDMPYLPDG TPVDIVLNPL
GVPSRMNVGQ VLETHLGIAA KKLGFNVATP VFDGVKEEKI REFLVKAGMD EDGKSILYDG
RTGEMFNQRV VVGMIYMMKL NHMVADKIHA RAVGPYSLVT QQPLGGKAQY GGQRFGEMEV
WAMEAYGAAY ILQELLTVKS DDVQGRTRIY ESIVKGENYL ETTTPESFNV LIKELQGLCL
EVNIGQRSDV IGLSVKPTTP KALSGGEAA
