RPOB_METNO
ID RPOB_METNO Reviewed; 1374 AA.
AC B8IS78;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mnod_1901;
OS Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=460265;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J.,
RA Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium nodulans ORS 2060.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001349; ACL56890.1; -; Genomic_DNA.
DR RefSeq; WP_015928581.1; NC_011894.1.
DR AlphaFoldDB; B8IS78; -.
DR SMR; B8IS78; -.
DR STRING; 460265.Mnod_1901; -.
DR EnsemblBacteria; ACL56890; ACL56890; Mnod_1901.
DR KEGG; mno:Mnod_1901; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000008207; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1374
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000165813"
SQ SEQUENCE 1374 AA; 152881 MW; 26F68349BB9BE1BC CRC64;
MANTLVGRKR IRKFFGKIRE VAEMPNLIEV QKASYDQFLM VDEPEGGRPD EGLQSVFKSV
FPISDFSSTA LLEFVKYTFE APKYDVDECR QRGITFAAPL KVTLRLIVFD VDPDTGAKSV
KDIKEQDVYM GDMPLMTENG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAA
RIIPYRGSWL DVEFDAKDIV YARIDRKRKI PVTSLLFALG LDGEEILSTF YNRISYTRDG
ADWRVPYDAE RLKGFKASTD LIDADSGEVV LEAGKKLTAR SARQIAEKGV TALRATDEDL
IGQYIAEDMV NYKTGEIYAE AGDEISEKLL KGLADVGIEE IPVLDIDHVN VGPYIRNTLA
VDKNSNREGA LFDIYRVMRP GEPPTLDTAE AMFHSLFFDA ERYDLSAVGR VKMNMRLDLD
APDTMRTLRR DDMLAVVKAL VDLRDGKGEI DDIDHLGNRR VRSVGELMEN QYRLGLLRME
RAIKERMSSV DIDTVMPQDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EVTHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATFARVN KYGFIETPFR
RVKNGVVTDE VVYLSAMEEA RYYVAQASAA MDENRRLTED LVVCRRAGEV IVVAPDRVDL
MDVSPKQLVS VAAALIPFLE NDDANRALMG SNMQRQAVPL VRADAPFVGT GMESVVARDS
GAAIAARRSG IVDQVDATRI VIRATEETDP TKPGVDIYRL QKFQRSNQST CITQKPLVRV
GEQVGKGDII ADGPSTEFGE LALGRNVLVA FMPWNGYNFE DSILLSERIV KDDVFTSIHI
EEFEVMARDT KLGPEEITRD IPNVSEEALK NLDEAGIVYI GAEVHAGDIL VGKITPKGES
PMTPEEKLLR AIFGEKASDV RDTSLRVPPG VTGTIVEVRV FNRHGVDKDE RAQAIEREEI
ERLAKDRDDE QAILDRNTYA RLADVLVGQA PVAGPKGFKK DTVLTREGLA DYPRSQWWQF
AVVDDRLMTE IEAMQKQYDE SKKRLEQRFL DKVEKLQRGD ELPPGVMKMV KVFVAVKRKI
QPGDKMAGRH GNKGVVSRIV PIEDMPFLED GTHADIVLNP LGVPSRMNVG QILETHLGWA
AAGLGRQVAQ AVDAYLRSHD ATPLREQMRR IYSEAELDGL SDQDLAEIGN NLRRGVPMAT
PVFNGAKEAD IEAMLEMAGL DRSGQSTLYD GRTGEPFDRK VTVGYIYMLK LHHLVDDKIH
ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVAGRTKV
YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVELISSKR AANDQLEAPP EAAE
