RPOB_METPB
ID RPOB_METPB Reviewed; 1375 AA.
AC B1ZGS0;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mpop_4499;
OS Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
OS (Methylobacterium populi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=441620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-705 / NCIMB 13946 / BJ001;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Marx C., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium populi BJ001.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001029; ACB82598.1; -; Genomic_DNA.
DR RefSeq; WP_012456202.1; NC_010725.1.
DR AlphaFoldDB; B1ZGS0; -.
DR SMR; B1ZGS0; -.
DR STRING; 441620.Mpop_4499; -.
DR EnsemblBacteria; ACB82598; ACB82598; Mpop_4499.
DR KEGG; mpo:Mpop_4499; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000007136; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1375
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141711"
SQ SEQUENCE 1375 AA; 152750 MW; 8CD5A0571D09D0E4 CRC64;
MANTLVGRKR IRKFFGKIRE VAEMPNLIEV QKASYDQFLM VDEPEGGRAD EGLQSVFKSV
FPISDFASTA LLEFVRYTFE QPKYDVDECR QRGITFAAPL KVTLRLIVFD VDPDTGAKSV
KDIKEQDVYM GDMPLMTDNG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAA
RIIPYRGSWL DVEFDAKDIV HVRIDRKRKL PVTSLLFALG LDGEEILSTF YNRVSYERDG
ADWRVPFDAE RLKGFKASVD LIDADSGEVV LEAGKKLNAR NARLIGEKGT KFLKATDEDL
VGQYIAEDLV NAKTGEIWAE AGDEISEKLL KALDDVGVTE IPVLDIDHVN VGPYIRNTLA
VDKNSAREGA LFDIYRVMRP GEPPTLDTAE AMFHSLFFDA ERYDLSAVGR VKMNMRLDLD
AADTVRTLRR EDMLAVVKAL VDLRDGKGEI DDIDHLGNRR VRSVGELMEN QYRLGLLRME
RAIKERMSSV DIDTVMPQDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EVTHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATFARVN KYGFIETPFR
RVKDGVVTDE VAYLSAMEEA KYYVAQANAG MDAARKLTDD LVVCRRAGEV IVVAPDRVDL
MDVSPKQLVS VAAALIPFLE NDDANRALMG SNMQRQAVPL VRADAPFVGT GMEAVVARDS
GAAIAARRSG IVDQVDATRI VIRATEETDP TKPGVDIYRL QKFQRSNQST CITQKPLVRV
GEPVKKGEII ADGPSTEFGE LALGRNVLVA FMPWNGYNFE DSILLSERIV KDDVFTSIHI
EEFEVMARDT KLGPEEITRD IPNVSEEALK NLDEAGIVYI GAEVHAGDIL VGKITPKGES
PMTPEEKLLR AIFGEKASDV RDTSLRVPPG VTGTIVEVRV FNRHGVDKDE RAQAIEREEI
ERLAKDRDDE QTILDRNTYA RLAEVLIGQS PIAGPKGFRK DTTLTREGIS EYPRSQWWQF
AVVDDRLMTE IEAMQKQYDE SKKRLEQRFL DKVEKLQRGD ELPPGVMKMV KVFVAVKRKI
QPGDKMAGRH GNKGVVSRIV PIEDMPFLED GTHADIVLNP LGVPSRMNVG QILETHLGWA
AAGLGRKVSK AVDAYLKNQD IAPLRAEMEA IYSPSELEGL SDEALAEAGN NVRRGVPMAT
PVFNGAKEAD IETMLEMAGL DRSAQSTLYD GRTGEPFDRK VTMGYIYMLK LHHLVDDKIH
ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVAGRTKV
YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVELTNSKQ QAANDQIEPP ADAAE
