ID   RPOB_METRJ              Reviewed;        1374 AA.
AC   B1LY43;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=Mrad2831_3839;
OS   Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 /
OS   NBRC 15690 / NCIMB 10815 / 0-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=426355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium radiotolerans JCM
RT   2831.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001001; ACB25814.1; -; Genomic_DNA.
DR   RefSeq; WP_012320772.1; NC_010505.1.
DR   AlphaFoldDB; B1LY43; -.
DR   SMR; B1LY43; -.
DR   STRING; 426355.Mrad2831_3839; -.
DR   EnsemblBacteria; ACB25814; ACB25814; Mrad2831_3839.
DR   KEGG; mrd:Mrad2831_3839; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000006589; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1374
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000141712"
SQ   SEQUENCE   1374 AA;  153094 MW;  D90AAB07D199958A CRC64;
     MANTLVGRKR IRKFFGKIKE VAEMPNLIEV QKASYDQFLM VDEPEGGRAD EGLQSVFKSV
     FPISDFASTA LLEFVRYTFE APKYDVDECR QRGITFAAPL KVTLRLIVFD VDPDTQAKSV
     KDIKEQDVYM GDMPLMTENG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAA
     RIIPYRGSWL DVEFDAKDIV HVRIDRKRKL PATSLLYALG LDGEEILSTF YNKVVYDRDG
     ADWRVPFDAE RMKGMKATVD LIDADSGEVV LEAGKKLNAR NARQITEKGT KFLKATDEDL
     VGQYVAEDLV NAQTGEIWAE AGEEVTDKLL KSLEEVGITE LPVLDIDHVN IGPYIRNTLA
     VDKNSNREGA LFDIYRVMRP GEPPTLETAE AMFHSLFFDS ERYDLSAVGR VKMNMRLDLD
     AADTVRTLRK EDMLAVVKAL VELRDGKGEV DDIDHLGNRR VRSVGELMEN QYRLGLLRME
     RAIRERMSQV DIDTVMPQDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EVTHKRRLSA
     LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATFARVN KYGFIETPFR
     RVRDGVVTDE VAYLSAMEEA KYYVAQANAQ MDENRKLTED LVVCRRAGEV IVVGPERVDL
     MDVSPKQLVS VAAALIPFLE NDDANRALMG SNMQRQAVPL VRADAPFVGT GMEAVVARDS
     GAAIAARRAG IIDQVDATRI VIRATEEADA NKPGVDIYRL QKFQRSNQST CITQKPLVRV
     GEFVKKGEII ADGPSTEFGE LALGRNVLVA FMPWNGYNFE DSILLSERIV KDDVFTSIHI
     EEFEVMARDT KLGPEEITRD IPNVSEEALK NLDEAGIVYI GAEVNAGDIL VGKITPKGES
     PMTPEEKLLR AIFGEKASDV RDTSLRVPPG VTGTIVEVRV FNRHGVDKDE RAQAIEREEI
     ERLAKDRDDE QAILDRNTYA RLADVLIGQA PVAGPKGFKK DTTLTRELIN DYPRSQWWQF
     AVIDDRLMTE MEAMQKQYDE SKKRLEQRFL DKVEKLQRGD ELPPGVMKMV KVFVAVKRKI
     QPGDKMAGRH GNKGVVSRIV PIEDMPFLED GTHADIVLNP LGVPSRMNVG QILETHLGWA
     AAGLGRKVSK AVDAYLKTQD IAPLREEMKA IYSPGELDGL TDEELAEAGN NVRRGVPMAT
     PVFNGAKEAD IEQMLEMAGL DRSAQSTLYD GRTGEPFDRK VTMGYIYMLK LHHLVDDKIH
     ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVAGRTKV
     YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVELTSSKK AANDQLEPPA DAAE