RPOB_METS4
ID RPOB_METS4 Reviewed; 1374 AA.
AC B0UHX6;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=M446_0360;
OS Methylobacterium sp. (strain 4-46).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=426117;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4-46;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Ivanova N., Marx C.J., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium sp. 4-46.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000943; ACA14931.1; -; Genomic_DNA.
DR RefSeq; WP_012330349.1; NC_010511.1.
DR AlphaFoldDB; B0UHX6; -.
DR SMR; B0UHX6; -.
DR STRING; 426117.M446_0360; -.
DR EnsemblBacteria; ACA14931; ACA14931; M446_0360.
DR KEGG; met:M446_0360; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1374
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141713"
SQ SEQUENCE 1374 AA; 152952 MW; 3F458941D9AA5EA1 CRC64;
MANTLVGRKR IRKFFGKIRE VAEMPNLIEV QKASYDQFLM VDEPEGGRPD EGLQSVFKSV
FPISDFSSTA LLEFVKYTFE APKYDVDECR QRGITFAAPL KVTLRLIVFD VDPDTGAKSV
KDIKEQDVYM GDMPLMTENG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAA
RIIPYRGSWL DVEFDAKDIV YARIDRKRKI PVTSLLFALG LDGEEILSTF YNRITYARDG
RDWRVPYDAE RLKGFKATTD LIDADSGEVV LEAGKKLTAR AARQIAEKGT KALRATDEDL
VGQYIAEDMV NYKTGEIYAE AGDEISEKLL KGLSDVGIEE IPVLDIDHVN VGPYIRNTLA
VDKNSAREGA LFDIYRVMRP GEPPTLDTAE AMFHSLFFDA ERYDLSAVGR VKMNMRLDLD
APDTMRTLRR EDMLAVVKAL VDLRDGKGEI DDIDHLGNRR VRSVGELMEN QYRLGLLRME
RAIKERMSSV DIDTVMPQDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EVTHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATFARVN KYGFIETPFR
RVRGGVVTDE VVYLSAMEEA KYYVAQASAA MDAERRLTED LVVCRRAGEV IVVAPDRVDL
MDVSPKQLVS VAAALIPFLE NDDANRALMG SNMQRQAVPL VRADAPFVGT GMEAVVARDS
GAAIAARRAG VIDQVDATRI VIRATEETDP TKPGVDIYRL QKFQRSNQST CITQKPLVRV
GEVVKKGDII ADGPSTEFGE LALGRNVLVA FMPWNGYNFE DSILLSERIV KDDVFTSIHI
EEFEVMARDT KLGPEEITRD IPNVSEEALK NLDEAGIVYI GAEVHAGDIL VGKITPKGES
PMTPEEKLLR AIFGEKASDV RDTSLRVPPG VTGTIVEVRV FNRHGVDKDE RAQAIEREEI
ERLAKDRDDE QAILDRNTYA RLADVLVGQS PVAGPKGFKK DTLLTREVLA DYPRSQWWQF
AVVDDRMMTE IEAMQKQYDE SKKRLEQRFL DKVEKLQRGD ELPPGVMKMV KVFVAVKRKI
QPGDKMAGRH GNKGVVSRIV PIEDMPFLED GTHADIVLNP LGVPSRMNVG QILETHLGWA
AAGLGRQVAQ AVDAYLRNQD AQPLREQMSR IYSPSELEGL SDRELAEVGN NLRRGVPMAT
PVFNGAKEAD IEAMLEMAGL DRSGQSTLYD GRTGEPFDRK VTVGYIYMLK LHHLVDDKIH
ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVAGRTKV
YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVELISSKR AANDQLEAPP EAAE
