ID   RPOB_MORIN              Reviewed;        1070 AA.
AC   Q09X25;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=MoinCp013;
OS   Morus indica (Mulberry).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Moraceae; Morus.
OX   NCBI_TaxID=248361;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. K2;
RA   Ravi V., Khurana J.P., Tyagi A.K., Khurana P.;
RT   "The chloroplast genome of mulberry: structural features and comparative
RT   analysis.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ226511; ABB20950.1; -; Genomic_DNA.
DR   RefSeq; YP_762253.1; NC_008359.1.
DR   AlphaFoldDB; Q09X25; -.
DR   SMR; Q09X25; -.
DR   PRIDE; Q09X25; -.
DR   GeneID; 4290596; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276592"
SQ   SEQUENCE   1070 AA;  121066 MW;  253AE4A3D4DF5383 CRC64;
     MLEGGNEEIS TIPGFNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLIKERDAV YESLTYSSEL YVSAGLIWKT SRDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRLEL EIDRKARIWA RVSRKQKISI
     LVLLSAMGLN LREILENVCY PEIFLSFLKD KEKKKIKSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNRRL NLDIPQNTTF LLPRDILAAA DHLIGMKFGM
     GILDDMNHLK NKRIRSVADL LQDQFGLALV RLENMVRGTM CGAIRHKLIP TPQNLVTSTT
     LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKASYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HARIGHWGSL ESPFYEISER SKKVRMLYLS PSIDEYYMVA
     AGNSLALNRG SQEEQVVPAR YRQEFLTIEW EQVHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSRSEK CIVGTGLECQ VALDSGVPTI AEHQGKIIYT DTEKIILSGN
     RDTLSIPLVI YQRSNKNTCM HQKPQVSRGK CIKKGQILAD GAATVGGELA LGKNVLVAYM
     PWEGYNSEDA VLINERLVYE DIYTSFHIRK YEIHTHVTSH GPERITNEIP HLEAHLLRNL
     DKNGIVMLGS WVETGDILVG KLTPQMAKES SYAPEDRLLR AILGIQISIS KETCLKLPIG
     GRGRVIDVRW IQKKGGSSYN PETIRVYISQ KREIKVGDKV AGRHGNKGIV SKILPRQDMP
     YLQDGRPVDM VFNPLGVPSR MNVGQIFECS LGLAGGLLDR HYRIAPFDER YEQEASRKLV
     FSELYEASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEQPV IIGKPYILKL IHQVDDKIHG
     RCSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIRARQEVL
     GTTMVGGPIP KPEDAPESFR LLVRELRSLA LELNHFLVSE KNFQINRKDV