RPOB_MYCGA
ID RPOB_MYCGA Reviewed; 1390 AA.
AC P47715;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=MYCGA2130;
GN ORFNames=MGA_1000;
OS Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
OS (Mycoplasmoides gallisepticum).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=710127;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A5969Var.B;
RX PubMed=8714123;
RA Skamrov A.V., Rozovskaia T.A., Gol'dman M.A., Feotistova E.S.,
RA Bibilashvili R.S.;
RT "Determination of the nucleotide sequence of the part of the Mycoplasma
RT gallisepticum genome, containing the rpoB gene, during the use of the Bal-
RT pTM method for obtaining sequential deletions of the sequenced fragment.";
RL Mol. Biol. (Mosk.) 30:61-75(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R(low / passage 15 / clone 2);
RX PubMed=12949158; DOI=10.1099/mic.0.26427-0;
RA Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F.,
RA Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.;
RT "The complete genome sequence of the avian pathogen Mycoplasma
RT gallisepticum strain R(low).";
RL Microbiology 149:2307-2316(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; L38402; AAB40951.1; -; Genomic_DNA.
DR EMBL; AE015450; AAP56563.2; -; Genomic_DNA.
DR RefSeq; WP_011113454.1; NC_004829.2.
DR AlphaFoldDB; P47715; -.
DR SMR; P47715; -.
DR PRIDE; P47715; -.
DR GeneID; 57203527; -.
DR KEGG; mga:MGA_1000; -.
DR HOGENOM; CLU_000524_4_1_14; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001418; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1390
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047918"
FT REGION 556..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 353
FT /note="S -> A (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 454
FT /note="D -> H (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 575
FT /note="S -> G (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 701
FT /note="R -> T (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 747
FT /note="D -> V (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 937
FT /note="T -> I (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
FT CONFLICT 990
FT /note="A -> V (in Ref. 1; AAB40951)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1390 AA; 156053 MW; 4E1004A6135074BD CRC64;
MKKNSAFHNT QYSPKVVRRD YSKVHNKYNP LNLPGIQVNA YKQFLEKELE EIIGSYFPIK
SPNGKYSVEF HGMKILDPQI TKEEASAESK TYDASLYVNL SLVNHQTGTV KKISKKSAKS
KAEGIYFADI PLMTENGAFI VNGIEKFVVA QIVRSPGAYI LNKSQVKLSN SRKRNQEGYI
CEVFPSKGTL MLFYIAENKD FVQAVVRDVG GESAKVFSIT TLLKAFGLSE IKIKEIFNNN
DYILRSLESE FYNEKQILNE ADIAQLIRDI ESDRISKVKS LPIDQKWKNL VLDWYKLNQE
KQELLNSSNP NPTKIESLNT HIGVVLRKLI CEKAAKHVIQ ELSISTRSLD NVSQKEEISY
QSILLQHFFQ KKRYDLSSAG RHKFVRKLRV SERLYQRTIA QDIKDLDGNV VIKQGTLMLK
EQLDLFKRLS KEKRLDIIKQ IDFINPELNT QFTDVNTYEE VQIYVNNDLR NETTQIIGID
GSNESIETLT LADLISIVSY IVNLPFNIGL YDDIDHLGNK RLKLINELLK SKVQTGMMRI
EKYIKDKLQT ADGNNKLADQ DAENDPDSDL GTKSSNDLTV KSVINPKPFQ IVIKDFFNTH
QLTQFLDQQN PLSELTNKRR ISAMGPGGIS REDPNLDIRD VHYSHYGRIC PIETPEGMNI
GLIMSLAFYA TIDKNGFLMT PYLKVENGKI TDKVEHLTAL REDEYIIAEA SSYMDVAKDG
TINNEKIIAR YRSSQDLYDP KQVDYIDVSP KQVVSVAASL IPFLENDDSS RALMGANMQR
QATPLLKPYS PLVGTGVEYK IAQDSGMLVT AKNPGTVTYV DASKITVKGE DNKSTDYNLL
KFVKSNKNTC YNQSPIVAVG DKIETNQALA DGPSMKNGEL ALGQNVLVGF TTWSGYNYED
AVIISDRLFK DDVYSSIHID EYTIECLRTK NGDEEITRDI PNISESAKRY LDEEGVIMVG
AEVKEGDILV GKTSPKGQVE LTPEEKLIQA ILGDKVKQVR ESSLKVPNGG DGIVAGIKRF
SIANGDELED DVLELVKVYV VQKRKIQIGD KVAGRHGNKG IISKIVAQED MPHLEDGTPL
DILLNPLGVP SRMNIGQIFE LHLGYAAREL AKAKLIEACF DKKLADQLDT VFGLEKSKTQ
SLIKNLVEHM KSIGVTSLAQ TKNRVRTIDI DIVLKQLGLT YDDLAFKIAT PVFEGVNMED
LKAIMSEAGI DPDKTEGKFK LIDGRTGEPF EKPISVGIMY MLKLDHMVDD KIHARAVGPY
SKITQQPLGG KSQNGGQRFG EMEVWALQAY GAAHNLREIL TIKSDDVRGR NMTYNAIVKG
LSIPEPGVPE SFKLLTKELQ GLGMTLNVLY DDDSIENINN ISVVDESLEP KIHDAEFDTF
TLDDYNDDNF
