RPOB_MYCGI
ID RPOB_MYCGI Reviewed; 1174 AA.
AC A4T1P4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mflv_5097;
OS Mycolicibacterium gilvum (strain PYR-GCK) (Mycobacterium gilvum (strain
OS PYR-GCK)).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=350054;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PYR-GCK;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.;
RT "Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000656; ABP47563.1; -; Genomic_DNA.
DR RefSeq; WP_011895920.1; NC_009338.1.
DR AlphaFoldDB; A4T1P4; -.
DR SMR; A4T1P4; -.
DR STRING; 350054.Mflv_5097; -.
DR PRIDE; A4T1P4; -.
DR EnsemblBacteria; ABP47563; ABP47563; Mflv_5097.
DR KEGG; mgi:Mflv_5097; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_11; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1174
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000086374"
SQ SEQUENCE 1174 AA; 129230 MW; ACBB1E6B62319DE3 CRC64;
MLEGCILAGS RQIESTTNNS VPGAPNRISF AKLREPLEVP GLLDVQTESF EWLIGAEDWF
QRAVDRGDVD PKGGLQEVLE ELSPIEDFSG SMSLSFSDPR FDEVKAPVDE CKDKDMTYAA
PLFVTAEFIN NNTGEIKSQT VFMGDFPMMT EKGTFIINGT ERVVVSQLVR SPGVYFDESI
DKSTEKTLHS VKVIPGRGAW LEFDVDKRDT VGVRIDRKRR QPVTVLLKAL GWTNEQIVER
FGFSEIMMST LEKDNTAGTD EALLDIYRKL RPGEPPTKES AQTLLENLFF KEKRYDLARV
GRYKVNKKLG LNVGQPITSS TLTEEDVVAT IEYLVRLHQG DQTMTAPGGS EVPVEVDDID
HFGNRRLRTV GELIQNQIRV GLSRMERVVR ERMTTQDVEA ITPQTLINIR PVVAAIKEFF
GTSQLSQFMD QNNPLSGLTH KRRLSALGPG GLSRERAGLE VRDVHSSHYG RMCPIETPEG
PNIGLIGSLS VYARVNPFGF IETPYRKVVD GVVSDQIDYL TADEEDRHVV AQANSPLDGD
GRFEEERVLV RRKGGEVEFV SASEVDYMDV SPRQMVSVAT AMIPFLEHDD ANRALMGANM
QRQAVPLVRS EAPLVGTGME LRAAIDAGDV VVTDKAGVVE EVSADYITVM ADDGTRHTYR
MRKFARSNHG TCANQRPIVD AGQRVETGQV LADGPCTENG EMALGKNLLV AIMPWEGHNY
EDAIILSNRL VEEDVLTSIH IEEHEIDARD TKLGAEEITR DIPNVSDEVL ADLDERGIIR
IGAEVRDGDI LVGKVTPKGE TELTPEERLL RAIFGEKARE VRDTSLKVPH GESGKVIGIR
VFSREDDDEL PAGVNELVRV YVAQKRKISD GDKLAGRHGN KGVIGKILPV EDMPFLPDGT
PVDIILNTHG VPRRMNIGQI LETHLGWVAK AGWNIRLASD GADGSTEVPA WAAKLPEHML
SAPADSIVAT PVFDGAQEGE LQGLLGATLP NRDGETMVNS DGKAVLFDGR SGEPFPYPVT
VGYMYILKLH HLVDDKIHAR STGPYSMITQ QPLGGKAQFG GQRFGEMECW AMQAYGAAYT
LQELLTIKSD DTVGRVKVYE AIVKGENIPE PGIPESFKVL LKELQSLCLN VEVLSSDGAA
IEMRDGDDED LERAAANLGI NLSRNESASV EDLA
