ID   RPOB_MYCMM              Reviewed;        1176 AA.
AC   B2HSJ3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=MMAR_0995;
OS   Mycobacterium marinum (strain ATCC BAA-535 / M).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=216594;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-535 / M;
RX   PubMed=18403782; DOI=10.1101/gr.075069.107;
RA   Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K.,
RA   Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C.,
RA   Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K.,
RA   Lord A., Moule S., Mungall K., Norbertczak H., Quail M.A.,
RA   Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R.,
RA   Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T.;
RT   "Insights from the complete genome sequence of Mycobacterium marinum on the
RT   evolution of Mycobacterium tuberculosis.";
RL   Genome Res. 18:729-741(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000854; ACC39451.1; -; Genomic_DNA.
DR   AlphaFoldDB; B2HSJ3; -.
DR   SMR; B2HSJ3; -.
DR   STRING; 216594.MMAR_0995; -.
DR   EnsemblBacteria; ACC39451; ACC39451; MMAR_0995.
DR   KEGG; mmi:MMAR_0995; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_11; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000001190; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1176
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000141714"
FT   REGION          13..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        16..34
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1176 AA;  129548 MW;  185C69A75D61A000 CRC64;
     MLEGRILADF RQTDASLHQG RPQSSSNSSV PGAPNRVSFA KLREPLEVPG LLDVQTDSFE
     WLIGSQRWRE SAAQRGDATP VGGLEEVLYE LSPIEDFSGS MSLSFSDPRF DEVKAPVDEC
     KDKDMTYAAP LFVTAEFINN NTGEIKSQTV FMGDFPMMTE KGTFIINGTE RVVVSQLVRS
     PGVYFDETID KSTDKLLHSV KVIPSRGAWL EFDVDKRDTV GVRIDRKRRQ PVTVLLKALG
     WSNEQIHERF GFSEIMMGTL EKDNTAGTDE ALLDIYRKLR PGEPPTKESA QTLLENLFFK
     EKRYDLARVG RYKVNKKLGL NAGQPITSST LTEEDVVATI EYLVRLHEGQ TAMTAPGGVE
     VPVETDDIDH FGNRRLRTVG ELIQNQIRVG MSRMERVVRE RMTTQDVEAI TPQTLINIRP
     VVAAIKEFFG TSQLSQFMDQ NNPLSGLTHK RRLSALGPGG LSRERAGLEV RDVHPSHYGR
     MCPIETPEGP NIGLIGSLSV YARVNPFGFI ETPYRKVVDG VVSDEIHYLT ADEEDRHVVA
     QANSPIDAQG RFVEPRVLVR RKAGEVEYVP SSEVDYMDVS PRQMVSVATA MIPFLEHDDA
     NRALMGANMQ RQAVPLVRSE APLVGTGMEL RAAIDAGDVV VADKAGVIEE VSADYITVMA
     DDGTRHTYRM RKFARSNHGT CANQSPIVDA GERVEAGQVI ADGPCTQNGE MALGKNLLVA
     IMPWEGHNYE DAIILSNRLV EEDVLTSIHI EEHEIDARDT KLGAEEITRD IPNVSDEVLA
     DLDERGIVRI GAEVRDGDIL VGKVTPKGET ELTPEERLLR AIFGEKAREV RDTSLKVPHG
     ESGKVIGIRV FSREDDDELP AGVNELVRVY VAQKRKISDG DKLAGRHGNK GVIGKILPAE
     DMPFLPDGTP VDIILNTHGV PRRMNIGQIL ETHLGWVAKS GWNIDVANGV PEWAGKLPEN
     LLSAQPDSIV STPVFDGAQE AELQGLLSAT LPNRDGEVLV DGDGKAKLFD GRSGEPFPYP
     VTVGYMYIMK LHHLVDDKIH ARSTGPYSMI TQQPLGGKAQ FGGQRFGEME CWAMQAYGAA
     YTLQELLTIK SDDTVGRVKV YEAIVKGENI PEPGIPESFK VLLKELQSLC LNVEVLSSDG
     AAIELREGED EDLERAAANL GINLSRNESA SVEDLA