RPOB_MYCMS
ID RPOB_MYCMS Reviewed; 1291 AA.
AC Q6MRX6;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=MSC_1008;
OS Mycoplasma mycoides subsp. mycoides SC (strain PG1).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PG1;
RX PubMed=14762060; DOI=10.1101/gr.1673304;
RA Westberg J., Persson A., Holmberg A., Goesmann A., Lundeberg J.,
RA Johansson K.-E., Pettersson B., Uhlen M.;
RT "The genome sequence of Mycoplasma mycoides subsp. mycoides SC type strain
RT PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP).";
RL Genome Res. 14:221-227(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; BX293980; CAE77615.1; -; Genomic_DNA.
DR RefSeq; NP_975973.1; NC_005364.2.
DR AlphaFoldDB; Q6MRX6; -.
DR SMR; Q6MRX6; -.
DR STRING; 272632.MSC_1008; -.
DR EnsemblBacteria; CAE77615; CAE77615; MSC_1008.
DR KEGG; mmy:MSC_1008; -.
DR PATRIC; fig|272632.4.peg.1095; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_14; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001016; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1291
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224079"
SQ SEQUENCE 1291 AA; 144828 MW; E3973E3F49FB30F6 CRC64;
MAYKIRKINR NVERRDYTKV SMNLSLPNLI GIQTETFEWF KTKGIQEVLD EFFPILSFDG
SSVLTLENWG FKEPRLSVRQ AKEESKIYDA PIYANLKLSV NKTEEIQKEF DGVVLDDALQ
ILSKWLEEKT VSKNITFKQQ SQNSYFFELT IKKSEKPDLI QIDIIEDKKT SLICNVSIYK
SGEVFLGDFP LMTEAGTFII NGSQKVIVSQ LVRSPGAYFN KELNRKTGEM IYFADIIPSR
GTWLEYETDS KKIGNDAINP LYVKIDKSRK TTATSLLLAF GISKDDILNI FDNDEVLVET
LQQDSIIGDF KIDWSNQVQE IYKKITQGET ATSEGASKFI NSILFDKRKY DLTKAGRFKL
KQKLSIKNRI LNRVIAEDIV DANNNVLIAK DTEVNKHNIK QISEILDQDV MSIDLNYLSD
IPGTRKVQKI KVYKDSELKT DTTCLIGLTS SSNEEFITVA DILSTVSYLL NLKYNIGEID
DIDNLGNRRV RTVGELLQNQ FRMGLNRIDK NVKEKLATSD LYKVKTSTII NAKPLTAIIG
EFFNLSQLSQ FMDQINPLSE LTNKRRLTAL GSGGLSRDRA GLEVRDVHPS HYGRICPIET
PEGPNIGLIN NLSTYARVNE YGFITTPYRK VINGIIQNDQ VEYLTADQEK NFIIAQSNVN
QDENGKILDE IIVSRFNGDD YMAKVEEIDY IDVSPKQIVS VATSGIPFLE NDDANRALMG
ANMQRQAVPL IKPESPIVAT GIEFEAARDS GEAIVAKEDA IVKYVDSKTI ITDGESGIRT
YILSDYERSN NGTSLTQSPI VKVGDVVKKG EIIADGPSMD QGELAIGQNV VVAFSTYNGY
NFEDAIVMSE RIVIDDRFTS THIDEYTLEV RNTKQGQEEV IREIPNMSEQ AKRHLDAEGI
VAIGTEVKVG DVLVGKVTPK GQVQLSPEDK LLHAIFGEKS RNVKDNSLRV PNGGEGIVQS
IKRFKAKSAL NPDGIELPAD IIEVIKVYVV QKRKIQEGDK MSGRHGNKGI ISRILPVEDM
PHLEDGTPVD IILNPQGVPS RMNIGQILEI HLGMAAKKLN QKVITPAFEG LNEKELEEIM
AEAGMTNYGK VTLIDGQTGE PFDKPIAVGV MYMLKLSHMV DDKIHARNVG PYSLITQQPL
GGKAQNGGQR FGEMEVWALE AYGAAHTLRE ILTIKSDDIK GRSKTYEAIV RSKKIPEPGI
PESFNVLSKE IMGLGFNMYM IDETGEKSVI NAYDKKNFDA DNYEDDEILV KTDTLYIDDE
DVDAEFEDLT YVDENDILRS FESENDIDEE E
