RPOB_NEIMA
ID RPOB_NEIMA Reviewed; 1392 AA.
AC P57009; A1IP03;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=NMA0142;
OS Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 /
OS Z2491).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=122587;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15465 / Z2491;
RX PubMed=10761919; DOI=10.1038/35006655;
RA Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M.,
RA Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M.,
RA Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K.,
RA Leather S., Moule S., Mungall K.L., Quail M.A., Rajandream M.A.,
RA Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G.,
RA Barrell B.G.;
RT "Complete DNA sequence of a serogroup A strain of Neisseria meningitidis
RT Z2491.";
RL Nature 404:502-506(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AL157959; CAM07460.1; -; Genomic_DNA.
DR PIR; D82007; D82007.
DR RefSeq; WP_002236600.1; NC_003116.1.
DR AlphaFoldDB; P57009; -.
DR SMR; P57009; -.
DR PRIDE; P57009; -.
DR EnsemblBacteria; CAM07460; CAM07460; NMA0142.
DR KEGG; nma:NMA0142; -.
DR HOGENOM; CLU_000524_4_3_4; -.
DR OMA; FMTWEGY; -.
DR BioCyc; NMEN122587:NMA_RS00720-MON; -.
DR Proteomes; UP000000626; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1392
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047929"
SQ SEQUENCE 1392 AA; 155576 MW; BA4AF438619CB82C CRC64;
MSYSFTEKKR IRKSFAKREN VLEVPFLLAT QIDSYAKFLQ LENAFDKRTD DGLQAAFNSI
FPIVSHNGYA RLEFVHYTLG EPLFDIPECQ LRGITYAAPL RARIRLVILD KEASKPTVKE
VRENEVYMGE IPLMTPSGSF VINGTERVIV SQLHRSPGVF FEHDKGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDLLYF RIDRRRKMPV TILLKALGYN NEQILDIFYD KETFYLSSNG
VQTDLVAGRL KGETAKVDIL DKEGNVLVAK GKRITAKNIR DITNAGLTRL DVEPESLLGK
ALAADLIDSE TGEVLASAND EITEELLAKF DINGVKEITT LYINELDQGA YISNTLRTDE
TAGRQAARVA IYRMMRPGEP PTEEAVEQLF NRLFFSEDSY DLSRVGRMKF NTRTYEQKLS
EAQQNSWYGR LLNETFAGAA DKGGYVLSVE DIVASIATLV ELRNGHGEVD DIDHLGNRRV
RSVGELTENQ FRSGLARVER AVKERLNQAE SENLMPHDLI NAKPVSAAIK EFFGSSQLSQ
FMDQTNPLSE VTHKRRVSAL GPGGLTRERA GFEVRDVHPT HYGRVCPIET PEGPNIGLIN
SLSVYARTND YGFLETPYRR VIDGKVTEEI DYLSAIEEGR YVIAQANADL DSDGNLIGDL
VTCREKGETI MATPDRVQYM DVATGQVVSV AASLIPFLEH DDANRALMGA NMQRQAVPCL
RPEKPMVGTG IERSVAVDSA TAIVARRGGV VEYVDANRVV VRVHDDEATA GEVGVDIYNL
VKFTRSNQST NINQRPAVKA GDVLQRGDLV ADGASTDLGE LALGQNMTIA FMPWNGYNYE
DSILISEKVA ADDRYTSIHI EELNVVARDT KLGAEDITRD IPNLSERMQN RLDESGIVYI
GAEVEAGDVL VGKVTPKGET QLTPEEKLLR AIFGEKASDV KDTSLRMPTG MSGTVIDVQV
FTREGIQRDK RAQSIIDSEL KRYRLDLNDQ LRIFDNDAFD RIERMIVGQK ANGGPMKLAK
GSEITTEYLA GLPSRHDWFD IRLTDEDLAK QLELIKVSLQ QKREEADELY EIKKKKLTQG
DELQPGVQKM VKVFIAIKRR LQAGDKMAGR HGNKGVVSRI LPVEDMPYMA DGRPVDIVLN
PLGVPSRMNI GQILEVHLGW AAKGIGERID RMLKEQRKAG ELREFLNRLY NGSGKKEDLD
ALTDEEIIEL ASNLRKGASF ASPVFDGAKE SEIREMLNLA YPSDDPEVEK LGFNDSKTQI
TLYDGRSGEA FDRKVTVGVM HYLKLHHLVD EKMHARSTGP YSLVTQQPLG GKAQFGGQRF
GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYENIVK GEHKIDAGMP ESFNVLVKEI
RSLGLDIDLE RY