ID   RPOB_NEOYE              Reviewed;        1143 AA.
AC   Q1XDN5;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Neopyropia yezoensis (Susabi-nori) (Pyropia yezoensis).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Neopyropia.
OX   NCBI_TaxID=2788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=U-51;
RA   Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T.,
RA   Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
RT   "Whole genome sequence of Porphyra yezoensis chloroplast.";
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP006715; BAE92376.1; -; Genomic_DNA.
DR   RefSeq; YP_536933.1; NC_007932.1.
DR   AlphaFoldDB; Q1XDN5; -.
DR   SMR; Q1XDN5; -.
DR   GeneID; 3978929; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1143
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000237328"
SQ   SEQUENCE   1143 AA;  128775 MW;  C83C9F7ECA68AA84 CRC64;
     MVQRISLKNK LLPDLVEIQR ASFKWFLLEG LTEVLEIFPK ISDPTSRLEL QLFGNEYKIK
     FPRYSVRQAK NRDKTYSAQI YVPAKLTRKD IDLPSKNQEK NIKSLDLSST HLQLSAEKQI
     KNKKYKKRLV FIGDLPIMTN RGTFIVSGTE RVIINQIIRS PGIYYKQEID KNGKQIYSAS
     LISNRGSWLK FEIDPKGEIW IRIDKTHKVN AYIFLRAIGL NKDEIEKGLS KYAFLISASQ
     IYSVKELAKE IGKNNIEEVT DEEALLIVYS KLRPNEPATV AVAKQMLYSR FFDPKRYDLG
     EVGRYKINKK LGLNIPKTFR VLSPQDILSS IDYLINIKDK NSGNLDDIDH LGNRRVRSVG
     ELLQNQFRVG LNRLERIIRE RMMICDIDSL SLSNLINPKP LIASVREFFG SSQLSQFMDQ
     TNPVAELTHK RRISALGPGG FNKDRAGFAV RDLHPSHYGR ICPIETPEGP NAGLIGSLAT
     CARVNVFGFI ETPFYPVNQG QVIYHNSPVY LTADEEDDFR VAPGDVKVSK QHYIEGDIIP
     VRYRQEFITT TPTQVDYIAI SPIQVISAAT SLIPFLEHDD ANRALMGSNM QRQAVPLLYP
     EKPIIGTGLE TKIARDSGMV VISRTSGHVN YVSANKIGIQ DNSGRTVHYR LKKYYRSNQD
     TCINQRPIVW VGEKIVVGQT LADGASTDGG EIALGRNILV AYMPWEGYNY EDAFLISERL
     VYDDLYTSIH IEKYEVECRQ TKLGPEEITR EIPNVSDNSL KDLDRNGIVV GGSWVEAGDI
     LVGKITPKGE ADQLPEGKLL RAIFGEKARD VRDTSLRLPN AAKGRVVKVR VFTRQKGDEL
     PPGTNAMIRV YVAQKRKIQV GDKMAGRHGN KGIISRILPK QDMPYLSDGT PVDIVLNPLG
     VPSRMNVGQV FECLLGLAGG YLGKRFKIIP FDEMYGAEAS RALVNRKLKE ASLITSNKWL
     FNDQHPGKMQ VFDGRTGEPF DNPVTVGRAY MLKLVHLVDD KIHARSTGPY SLVTQQPLGG
     RAQHGGQRLG EMEVWALEAF GAAYTLQELL TVKSDDMQAR NEALNAIVKG KPIPKPGTPE
     SFKVLMRELQ SLGLDIAVHK LKLFEDGQRR TVEVDLMSDS KDNRVDRSNY DTPPVDDFEQ
     FLY