ID   RPOB_NEPOL              Reviewed;        1109 AA.
AC   Q9TL06;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Nephroselmis olivacea (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Nephroselmidophyceae;
OC   Nephroselmidales; Nephroselmidaceae; Nephroselmis.
OX   NCBI_TaxID=31312;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-484 / S-N-5-8;
RX   PubMed=10468594; DOI=10.1073/pnas.96.18.10248;
RA   Turmel M., Otis C., Lemieux C.;
RT   "The complete chloroplast DNA sequence of the green alga Nephroselmis
RT   olivacea: insights into the architecture of ancestral chloroplast
RT   genomes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:10248-10253(1999).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AF137379; AAD54810.1; -; Genomic_DNA.
DR   RefSeq; NP_050839.1; NC_000927.1.
DR   AlphaFoldDB; Q9TL06; -.
DR   SMR; Q9TL06; -.
DR   PRIDE; Q9TL06; -.
DR   GeneID; 801913; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1109
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048032"
SQ   SEQUENCE   1109 AA;  124886 MW;  C8118702BD4A5849 CRC64;
     MIQNLQRKDS QWTPFILPDL ISIQRDSFLL FLERGLATEL SHVTPLTGSH FRITLCSHGY
     RLKRPKVSIQ EAVYQATSYS AALYVNVETN QSNLGTQETQ IQSVWMGDIP LMTSRGHFII
     NGSSRVVVNQ IVRSPGIYFK EMIDQKHRRM FQASIISNRG SWVRLETDVD GMIWVRMDKA
     KKISILIVLE AMGLSKKTIF RSLKSPEFLF KALQQLLAKK RWQDKVYELI PQSTTDALFH
     LYTKLSPDKP GNTLTARRVL YEKLMDAKRY DVGLVGRVKL NKKLKLPVPE HVHTLRPVDF
     LAATDYLIGL EYGRGQVDDI DHLKNRRVRC AGELIQSQLR IGLNRLERTM QGRISRPGEL
     PGMSSLMNPK PVMAALREFF GSNPLSQFMD QTNPLAELTH KRRLSSLGPG GLSQDRAGMA
     VREIHPSQYG RICPIETPEG PNAGLIGSMA TYARLNQYGG LECPFYQVVE GKVLYEFGPI
     FLTAEQEDQV TVVAGDILDQ RKLMVAVAEG RDPLDPAVRP TLPDRPLILR SRQEFHTGSF
     RDVNYVGIAP TQMISVATSL IPFLEHDDAN RALMGSNMQR QAVPLLKTEA PIIGTGLEAQ
     VAADAGGVVQ SPFEGIVVYV DATRIVVSTS KSKLALSKKR KIHESNVFLQ VYQRSNQGTC
     IHQRPIIPLH TPVIRGDLLA DGSSTSGGQI ALGKNLLVAY MPWEGYNFED AILISERLIY
     DDLYTSLHIE RYEVETQHTK LGPEEITKSI VHETDDKLPY RWLDERGIVM RGAWVEPGDV
     LIGKITPKEE KELTPELRLV YAIFGKRPKG FRDTSLRVPQ GVRGRVVDVR MIRDEDTKVV
     HVYVCQQRQI QVGDKMAGRH GNKGIVSRIV PRQDMPYLQD GTPVDMVLNP LGVPSRMNVG
     QVFECLLGLA GQRLGQQLKV RAFDEMYGAE ASRHLVYNKL HEASEVTGHH WLFDPNHPGK
     SRLIDGRTGD MFDQAVTIGQ AYMLKLIHQV DDKIHARATG PYSLITQQPL GGRSKRGGQR
     LGEMEVWAFE GFGAAYTLQE LLTVKSDDMQ GRNEIMSAMV QGRQLPEMGT PESFKVMIRE
     LQALCLDIGI YHRSKMTFKT EEIDLMQMR