ID   RPOB_NICTO              Reviewed;        1070 AA.
AC   Q33C46;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Nicotiana tomentosiformis (Tobacco).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4098;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16435119; DOI=10.1007/s00438-005-0092-6;
RA   Yukawa M., Tsudzuki T., Sugiura M.;
RT   "The chloroplast genome of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris
RT   progenitor is the maternal genome donor of Nicotiana tabacum.";
RL   Mol. Genet. Genomics 275:367-373(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AB240139; BAE47989.1; -; Genomic_DNA.
DR   RefSeq; YP_398851.1; NC_007602.1.
DR   AlphaFoldDB; Q33C46; -.
DR   SMR; Q33C46; -.
DR   GeneID; 3776378; -.
DR   KEGG; nto:3776378; -.
DR   OrthoDB; 944344at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224130"
SQ   SEQUENCE   1070 AA;  120597 MW;  9C21F305DA00FC90 CRC64;
     MLGDGNEGIS TIPGFNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLIKERDAV YESLTYSSEL YVSAGLIWKN SRDMQEQTFF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
     LVLSSAMGLN LREILENVCY PEIFLSFLSD KERKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNRRL NLDIPQNNTF LLPRDILAAA DHLIGLKFGM
     GALDDMNHLK NKRIRSVADL LQDQFGLALV RLENVVRGTI CGAIRHKLIP TPQNLVTSTP
     LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKLSYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HAKIGHWGSL ESPFYEISER STGVRMLYLS PGRDEYYMVA
     AGNSLALNRD IQEEQVVPAR YRQEFLTIAW EQVHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSRSEK CIVGTGLERQ AALDSGALAI AEREGRVVYT NTDKILLAGN
     GDILSIPLVI YQRSNKNTCM HQKLQVPRGK CIKKGQILAD GAATVGGELA LGKNVLVAYM
     PWEGYNSEDA VLISERLVYE DIYTSFHIRK YEIQTHVTSQ GPEKVTNEIP HLEAHLLRNL
     DKNGIVMLGS WVETGDILVG KLTPQVVKES SYAPEDRLLR AILGIQVSTS KETCLKLLIG
     GRGRVIDVRW IQKRGGSSYN PETIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
     YLQDGRSVDM VFNPLGVPSR MNVGQIFECS LGLAGSLLDR HYRIAPFDER YEQEASRKLV
     FSELYEASKQ TANPWVFEPE YPGKSRIFDG RTGNPFEQPV IIGKPYILKL IHQVDDKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIRARQEVL
     GTTIIGGTIP NPEDAPESFR LLVRELRSLA LELNHFLVSE KNFQINRKEA