RPOB_NITEC
ID RPOB_NITEC Reviewed; 1359 AA.
AC Q0AF53;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Neut_1795;
OS Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=335283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 101675 / C91 / Nm57;
RX PubMed=17991028; DOI=10.1111/j.1462-2920.2007.01409.x;
RA Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S.,
RA Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.;
RT "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas
RT eutropha C91: implications for niche adaptation.";
RL Environ. Microbiol. 9:2993-3007(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000450; ABI60029.1; -; Genomic_DNA.
DR RefSeq; WP_011634835.1; NC_008344.1.
DR AlphaFoldDB; Q0AF53; -.
DR SMR; Q0AF53; -.
DR STRING; 335283.Neut_1795; -.
DR PRIDE; Q0AF53; -.
DR EnsemblBacteria; ABI60029; ABI60029; Neut_1795.
DR KEGG; net:Neut_1795; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001966; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1359
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300359"
SQ SEQUENCE 1359 AA; 151908 MW; A136803484D52488 CRC64;
MSYSFAEKKR IRKSFAKRTS ILPFPFLLAT QIESYADFLQ ADVAPGKRKN QGLQAAFNAV
FPIESHSNNA RLDFISYMLG TSVFDVKECQ QRGLTYASPL RARVRLTIMD KEASKPTVKE
VKEQEVYMGE IPLMTETGSF VVNGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDYVYF RIDRRRKMPV TTLLKAMGYS STQILADFFE FDHFTLAGNK
ILFHLIPERL RGELASFDIV SEDGKVFVQK DKRITAKHVR DLQQANLTKI SVPEEFLLGK
ILAEDIVDKE TGEVVATANS EINETLLERI KQTQNKNSEI STLFVNDLNY GPYISQTLRV
DESTDQMSAQ VAIYRMMRPG EPPTEDAVLA LFNGLFYSPE RYDLSVVGRM KFNRRVGREE
LTGSTTLSNE DITDVIKILV GLRNGRGEID DIDHLGNRRV RSVGELAENQ FRAGLARVEK
AVKERLSQAE SENLMPHDFI NAKPVSSAIR EFFGSSQLSQ FMDQTNPLSE ITHKRRISAL
GPGGLTRERA GFEVRDVHPT HYGRVCPIET PEGPNIGLIN SLALYARTNE YGFIETPYRM
VKNSRVTEEV VYLSAIEESQ YVIAQANANF DQTGVFTDEV VSCRHKNEFT LASRDQIEYV
DIAPAQIVSV AASLIPFLEH DDANRALMGS NMQRQAVPCL RAEKPLVGTG IERVVAVHSG
TAVKALRGGV VDYVDAGRIV IRVHDAETRA GEVGVDIYNL TKYIRSNQNT NINQRPIVKI
GDILSREDVI ADGASTDLGE LALGQNMLIA FMPWNGLNFE DSILISERVV SDDRFTSIHI
EELTAVSRDT KLGTEEVTAD IPNLSERQRA RLDESGIVYI GAEVEAGDVL VGKVTPKSET
QLTPEEKLLR AIFGEKASDV KDTSLHVPAG ISGTVIDVQI FTREGVDRDK RSKQIIADEL
GRFKKDLADQ MRIVEADAFQ RAKRLLAGKV AAGGPKKLAK NSTITQEYLE SVEKHHWFDI
RLIDENTSLQ LEQIKDSLVQ KRKLFDLAFE EKQRKLSQGD ELPPGVQKMV KVYIAVKRRL
QSGDKMAGRH GNKGVISKIV PVEDMPYMAD GTPVDVVLNP LGVPSRMNIG QVLEVHLGWA
AKGLGKKINE MLISQRDASE IRDFLSKIYS DNGKQEDLVS LDDKEILELA KNLSDGVPFA
TPVFDGAHES EIKHMLKLAD LPESGQTTLY DGRTGEAFDR LVTVGYMHVL KLHHLVDDKM
HARSTGPYSL VTQQPLGGKA QFGGQRFGEM EVWALEAYGA AYTLQEMLTV KSDDVNGRTK
VYESIVKGDH KIDAGMPESF NVLVKEIRSL GLDIDLEQH
