RPOB_NITEU
ID RPOB_NITEU Reviewed; 1357 AA.
AC Q82T75;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=NE2046;
OS Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS 14298).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=228410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT chemolithoautotroph Nitrosomonas europaea.";
RL J. Bacteriol. 185:2759-2773(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AL954747; CAD85957.1; -; Genomic_DNA.
DR RefSeq; WP_011112559.1; NC_004757.1.
DR AlphaFoldDB; Q82T75; -.
DR SMR; Q82T75; -.
DR STRING; 228410.NE2046; -.
DR PRIDE; Q82T75; -.
DR EnsemblBacteria; CAD85957; CAD85957; NE2046.
DR KEGG; neu:NE2046; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR PhylomeDB; Q82T75; -.
DR Proteomes; UP000001416; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1357
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047931"
SQ SEQUENCE 1357 AA; 151796 MW; F20CEECB12A09A79 CRC64;
MSYSFAEKKR IRKSFAKRAS ILPFPFLLAT QIQSYTDFLQ AEIAPGKRKN QGLQAAFNSV
FPIESHSNNA RLDFISYMLG SPVFDVKECQ QRGLTYAAPL RARVRLTILD KEASKPTVKE
VKEQEVYMGE IPLMTDTGSF VVNGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDYVYF RIDRRRKMPV TTLLKAMGYS PAQILADFFE FDHFILVDNK
IFFNLIPERL RGELAGFDIV SEDGKVFVQK DKRITAKHVR DLQQANLTKI PVPEEFLLGK
ILAADLVDKE TGEIIALANS EISETLLGRI RQTQSSEIST LFVNDLNYGP YISQTLRIDE
TTDQMSAQVA IYRMMRPGEP PTEEAVLALF NGLFYSPERY DLSVVGRMKF NRRVGREELT
GSTTLSNDDI IDVIKILVEL RNGRGEIDDI DHLGNRRVRS VGELAENQFR AGLARVEKAV
KERLSQAESE NLMPHDFINA KPVSSAIREF FGSSQLSQFM DQTNPLSEVT HKRRISALGP
GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL ALYARTNEYG FIETPYRMVR
NGRVTEEVVY LSAIEESQYV IAQANANFDQ NGVFTDEVVS CRHKNEFTLA SRDQIEYVDI
APAQIVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPCLRA EKPLVGTGIE RVVAVHSGTA
VRTIRGGVVD YVDASRIVIR VHDAEARAGE VGVDIYNLTK YTRSNQNTNI NQRPIVRMGD
VLSRDDVIAD GASTDLGELA LGQNMLIAFM PWNGLNFEDS ILISERVVSD DRFTSIHIEE
LAAVSRDTKL GTEEITADIP NLSERQRARL DESGIVYIGA EVEAGDVLVG KVTPKSETQL
TPEEKLLRAI FGEKASDVKD TSLHVPAGIS GTVIDVQIFT REGVDRDKRS KQIIADELGR
FKKDLADQMR IVEADAFQRA ERLLTGKVAA GGPKKLAKNS TITRDYLENV EKHHWFDIRL
VDENTSLQLE QIKDSLVQKR KLFDLAFEEK HRKLSQGDEL PPGVQKMVKV YIAVKRRLQS
GDKMAGRHGN KGVISKIVPI EDMPYMADGT PVDVVLNPLG VPSRMNIGQV LEVHLGWAAK
ELGKRVNEML ASQRNVSDIR DFLNKIYNNS GKQEDLTSLE DDEVLALARN LSSGVPFATP
VFDGAHESEI KQMLKLAGLP ESGQTTLYDG RTGEAFDRPV TVGYMHVLKL HHLVDDKMHA
RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGASY TLQEMLTVKS DDVNGRTKVY
ESIVKGDHKI DAGMPESFNV LVKEIRSLGL DIDLEEH