RPOB_NITHX
ID RPOB_NITHX Reviewed; 1380 AA.
AC Q1QN44;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Nham_1531;
OS Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Nitrobacter.
OX NCBI_TaxID=323097;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10229 / NCIMB 13809 / X14;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Ward B., Arp D., Klotz M., Stein L.,
RA O'Mullan G., Starkenburg S., Sayavedra L., Poret-Peterson A.T.,
RA Gentry M.E., Bruce D., Richardson P.;
RT "Complete sequence of chromosome of Nitrobacter hamburgensis X14.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000319; ABE62353.1; -; Genomic_DNA.
DR RefSeq; WP_011510042.1; NC_007964.1.
DR AlphaFoldDB; Q1QN44; -.
DR SMR; Q1QN44; -.
DR STRING; 323097.Nham_1531; -.
DR EnsemblBacteria; ABE62353; ABE62353; Nham_1531.
DR KEGG; nha:Nham_1531; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001953; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1380
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300358"
SQ SEQUENCE 1380 AA; 154219 MW; 7D1B2045EC393C5A CRC64;
MVQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVDEPEGGRL DEGLQAVFKS
VFPINDFSGT SQLEFVRYEF EAPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETAAKS
VKDIKEQDVY MGDIPLMTMN GTFVVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA
ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEEILST FYKKIIYKRA
KSGSNADGWR VPYDPVRFRG YSTLNDLIDA DSGKVVLEAG KKLTVRAARQ LQEKGLKALR
MSDEELVGMY LAEDLVNPKT GEIYAEAGEE ITEKSLKALN EHGYKELPLL DIDHVNVGPY
IRNTLSADKN LTREDALFDI YRVMRPGEPP TLESAQNMFQ SLFFDSERYD LSAVGRVKMN
MRLDLDAPDT YRTLRKEDIL AVIKTLVDLR DGKGEIDDID HLGNRRVRSV GELMENQYRV
GLLRMERAIK ERMSSVDIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH
KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF
VETPYRKVKD GRVTDEVVYL SAMEEGRYHV AQANLPLDNR GRFTEDLVVC RHAGEVLPVT
SDKVDFMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMEG
VVARDSGAAI AARRSGIIDQ IDATRVVIRA TEDLDPTKSG VDIYRLMKYQ RSNQSTCINQ
RPLVKVGDVV KKGDIIADGP STDLGELALG RNVLVAFMPW NGYNFEDSIL LSERIVKEDV
FTSIHIEEFE VMARDTKLGP EEITRDIPNV SEEALKNLDE AGIVYIGAEV RAGDILVGKI
TPKGESPMTP EEKLLRAIFG EKASDVRDTS LRVPPGVQGT IVEVRVFNRH GVDKDERALA
IEREEIERLA KDRDDEQAIL DRNVYGRLAD LLENRQGIAG PKGFKKDTKI TRSVIEEYPK
SQWWLFASPN DKLMAEIEAM RKQYDESKKG LEQRFLDKVE KLQRGDELPP GVMKMVKVFV
AVKRKIQPGD KMAGRHGNKG VVSKIVPIED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE
THLGWACAGL GRRIGQAVDA YLASAKQETK PLKETLKKVY GDNETIKSLN DHELVELGRN
LSRGVPIATP VFDGAKEADI EQMLELAGMD KSGQSTVYDG RTGDTFDRKV TVGYIYMLKL
HHLVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS
DDVAGRTKVY EAIVRGDDTF EAGIPESFNV LVKEMRSLGL NVDLHNSKIG SATPTSEAAE