RPOB_NITMU
ID RPOB_NITMU Reviewed; 1357 AA.
AC Q2YB05;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Nmul_A0759;
OS Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosospira.
OX NCBI_TaxID=323848;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25196 / NCIMB 11849 / C 71;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M.,
RA Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC
RT 25196.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000103; ABB74066.1; -; Genomic_DNA.
DR RefSeq; WP_011380116.1; NZ_FNVK01000003.1.
DR AlphaFoldDB; Q2YB05; -.
DR SMR; Q2YB05; -.
DR STRING; 323848.Nmul_A0759; -.
DR EnsemblBacteria; ABB74066; ABB74066; Nmul_A0759.
DR KEGG; nmu:Nmul_A0759; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002718; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1357
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000237307"
SQ SEQUENCE 1357 AA; 150941 MW; C8947063365C55B1 CRC64;
MSYSFTEKKR IRKSFAKRAS VLPIPFLLAT QIESYAAFLQ ANLEPDQRAP EGLQAAFLSI
FPIESHSRNA RLDFVNYVLG APPFDVKECQ QRGLTYASPL RAKVRLTIMD KEASKPTVRE
VKEQEVYMGE IPLMTNTGSF VINGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDYLYF RVDRRRKMPI TVLLKAIGYT PEQILENFFS FDSFHISRKS
ILFELLPERL RGDTARFDIL SKSGKVIVQK DKRITVKHVR EMQQAGIDRL EVPEDFLLGR
VLGHNVVDKE TGEILALAND EITETLLGKL RDANVEEIRT IYTNDLDQGA YISQTLKIDE
TADEMAAQVA IYRMMRPGEP PTEEAVKALF IGLFYAPERY DLSVVGRMKF NRRVGKTELD
GPTTLSNEDI IAVIRILVEL RNGRGEIDDI DHLGNRRVRS VGELAENQFR SGLVRVERAV
KERLSQAESE NLMPHDLINA KPVSAAVREF FGSSQLSQFM DQTNPLSEIT HKRRVSALGP
GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL ALYAQTNEYG FMETPYRKVT
DGLVTDEIHF LSAIEEGQYV IAQANAELDG DGRFTNDIVS CRHKNEFTLA TPDRIEYMDV
APAQIVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPCLRA EKPLVGTGIE RVVAVDSGTA
VKAVRGGVVD YVDASRIVVR VHDIETRAGE VGVDIYNLTK YTRSNQNTNI NQRPLVKVGD
VIARGDVVAD GASTDKGELA LGQNMLVAFM PWTGYNFEDS ILISERIVAN DRFTSIHIEE
LSVVSRDTKL GTEEITGDIS NLSEAQLGRL DASGIVHIGA EVEAGDVLVG KVTPKGETQL
TPEEKLLRAI FGEKASDVKD TSLRVPSGIS GTVIDVQVFT REGIERDKRA QQIIDDELKR
YKKDLADQMR IVEADAFVRI ERLLIGKIAT GGPKKLAKGA TLTKEYLESI DPHHWFDIRL
ADEGASVQLE QIEEGLAQKR KAFDAAFEEK KKKLTQGDEL PPGVQKMVKV YVAVKRRLQP
GDKMAGRHGN KGVISKIVPV EDMPYMADGT PVDVVLNPLG VPSRMNVGQI LETHLGWAAK
GLGQKIGDML RAGKEIVELR AFLDKIYNTS GKPEDIASLT DGEIADLAQN LQDGVPFATP
VFDGATEQEI KDMLELAGLP RSGQVTLHDG RTGEAFDRPV TVGYMHVLKL HHLVDDKMHA
RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGSAY TLQEMLTVKS DDVNGRTKVY
ESIVKGDHKI DAGMPESFNV LVKEIRSLAM DIDLERH
