RPOB_NITSB
ID RPOB_NITSB Reviewed; 1386 AA.
AC A6Q1M3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=NIS_0268;
OS Nitratiruptor sp. (strain SB155-2).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Nautiliales;
OC Nitratiruptoraceae; Nitratiruptor; unclassified Nitratiruptor.
OX NCBI_TaxID=387092;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB155-2;
RX PubMed=17615243; DOI=10.1073/pnas.0700687104;
RA Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K.,
RA Horikoshi K.;
RT "Deep-sea vent epsilon-proteobacterial genomes provide insights into
RT emergence of pathogens.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AP009178; BAF69382.1; -; Genomic_DNA.
DR RefSeq; WP_012081645.1; NC_009662.1.
DR AlphaFoldDB; A6Q1M3; -.
DR SMR; A6Q1M3; -.
DR STRING; 387092.NIS_0268; -.
DR EnsemblBacteria; BAF69382; BAF69382; NIS_0268.
DR KEGG; nis:NIS_0268; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_7; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001118; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1386
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329184"
SQ SEQUENCE 1386 AA; 157341 MW; FD40595E21C5E82B CRC64;
MLNSLQSGSR LRVDFSKIPQ ELDVPNLLQL QKSSYENFLM ADSKSRENSG IEKVFRSVFP
IHDPHNRISL EYAGSEIIKP KYTVRECMER GITYSVSLKM NIRLILWERD EKSGEKTGVK
DVKEQSIYVR DIPYMTDRTS FIINGVERVV VNQLHRSPGV IFKEEEASTS SGTMIHTAQI
IPDRGAWLYF EYDPKDILYV RINKRRKIPS TILFRALGYS KLDILKLFYP ITKIIIKENK
FFIEFRPEDF IGRVEFDVRD ENGNLIVEAG KRLTKKKAQK LIEEGVKYIE FPLDVLMDRH
LASPIIDQES GEVLYDTLTQ LDEHKLKKIL ELGIDEFEIV NDIASGKDRA IINSFIADQE
SLKLLKQTEG IEDENDLAAI RIYKVMRPGE PVTKETAKAF IQQLFFDPER YDITRVGRMK
MNHKLGLDVP EYVTVLTSED IIKTVQYLIK VKNGQGHIDD RDHLGNRRIR AIGELLANEL
HSGLVKMQKA IRDKMSTISG SLDELMPHDL INSKMITNTI LEFFATGQLS QFMDQTNPLS
EITHKRRLSA LGEGGLVKER AGFEVRDVHP THYGRICPIE TPEGQNIGLI NTLSTYAKVN
ELGFIEAAYK VVKDGKITDE IVYLTAAQEE GKIIAPANTE IIDGNEIKGD YVEARKDGEI
ILVEKNKVEL IDLTPRMVVG VAASLIPFLE HDDANRALMG SNMQRQAVPL LRTEAPIVGT
GMEKVVARDA WESIRARRSG VVEKIDSENI YILGEDENGA YIDHYRLQKN LRTNQNTCFT
QKPIVKKGQF VEAGQVITDG PNMDHAELAL GKNMLVAFMP WNGYNFEDAI VVSERILRDD
EFTSVHIYEK EIEARELKHG VEEITRDIPN VKEEEIEHLD ESGIVKIGTY VKPGMILVGK
VSPKGEVRPS PEERLLRAIF GEKAGHVVNK SLYCPQSMEG VVVDVKIFTK KGYDKDPRAI
KAYEEEKERL SKEHHDKLLM IDREEMLKII SLLSKEPLEK DAKIKDKEFK AGEKISKEEL
SQINRFALNA LVKSYAPEVQ KKYNAIKTHF QNEKRKLTEE HEEKLAILEK EDILPSGVVK
LVKVFIATKR KLKVGDKMAG RHGNKGIVSV IVPEIDMPYT KDGRIVDIVL NPLGVPSRMN
IGQILEVHLG LIGKKLGEQI QEIFEAKRAD FVKELRQKMI EIADVAKLMN AKETIEKMSD
EELIEYARDW SKGVKFATPV FEGVTAEEFE KLYELAKMDL DGKTELYDGR TGEKFKERVT
VGYMYMLKLH HLVDEKVHAR STGPYSLVTQ QPVGGKALFG GQRFGEMEVW ALEAHGAAHT
LKEMLTIKSD DVEGRVAAYK AITKGEPIPQ PGIPETLFVL TKELQSLGID VEILDEVKDD
EETGTN
