ID   RPOB_NOSS1              Reviewed;        1131 AA.
AC   P22703;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=alr1594;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 967-1131.
RX   PubMed=1904436; DOI=10.1128/jb.173.11.3446-3455.1991;
RA   Bergsland K.J., Haselkorn R.;
RT   "Evolutionary relationships among eubacteria, cyanobacteria, and
RT   chloroplasts: evidence from the rpoC1 gene of Anabaena sp. strain PCC
RT   7120.";
RL   J. Bacteriol. 173:3446-3455(1991).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC       alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC       factor is associated with the core the holoenzyme is formed, which can
CC       initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000019; BAB77960.1; -; Genomic_DNA.
DR   EMBL; M60831; AAA22032.1; -; Genomic_DNA.
DR   PIR; A42361; A42361.
DR   PIR; AD2005; AD2005.
DR   AlphaFoldDB; P22703; -.
DR   SMR; P22703; -.
DR   STRING; 103690.17135414; -.
DR   EnsemblBacteria; BAB77960; BAB77960; BAB77960.
DR   KEGG; ana:alr1594; -.
DR   eggNOG; COG0085; Bacteria.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1131
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047854"
FT   REGION          1108..1131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1131 AA;  126525 MW;  7AB6E1D28199FFD9 CRC64;
     MSSKKFNQVD KGRGMISENY IEPAFLLPDL IEIQRSSFRW FLEEGLIEEL NSFSPITDYT
     GKLELHFLGH NYKLKEPKYS VEEAKRRDST YAVQMYVPTR LLNKETGDIK EQEVFIGDLP
     LMTDRGTFII NGAERVIVNQ IVRSPGVYYK SEIDKNGRRT YSASLIPNRG AWLKFETDRN
     DLVWVRIDKT RKLSAQVLLK ALGLSDNEIF DALRHPEYFQ KTIEKEGQFS EEEALMELYR
     KLRPGEPPTV LGGQQLLDSR FFDPKRYDLG RVGRYKLNKK LRLSVPDTVR VLTSGDILAA
     VDYLINLEYD IGSIDDIDHL GNRRVRSVGE LLQNQVRVGL NRLERIIRER MTVSDAEVLT
     PASLVNPKPL VAAIKEFFGS SQLSQFMDQT NPLAELTHKR RLSALGPGGL TRERAGFAVR
     DIHPSHYGRI CPIETPEGPN AGLIGSLATH ARVNQYGFLE TPFRPVENGR VRFDQPAAYM
     TADEEDDLRV APGDIPVDEN GYIIGPQVPV RYRQEFSTTT PEQVDYVAVS PVQIVSVATS
     MIPFLEHDDA NRALMGSNMQ RQAVPLLKPE RPLVGTGLEA QGARDSGMVI VSRTDGDVVY
     VDATEIRVRV SGQLPTASGK STDNGQLTSQ KGQEIRYTVS KYQRSNQDTC LNQKPLVRIG
     ERVVAGQVLA DGSSTEGGEL ALGQNIVVAY MPWEGYNYED AILISERLVQ DDIYTSIHIE
     KYEIEARQTK LGPEEITREI PNVGEDALRQ LDEQGIIRIG AWVEAGDILV GKVTPKGESD
     QPPEEKLLRA IFGEKARDVR DNSLRVPNGE KGRVVDVRLF TREQGDELPP GANMVVRVYV
     AQKRKIQVGD KMAGRHGNKG IISRILPIED MPYLPDGSPV DIVLNPLGVP SRMNVGQVFE
     CLLGWAGHTL GVRFKITPFD EMYGEESSRR IVHGKLQEAR DETGKDWVYN PDDPGKIMVY
     DGRTGEAFDR PVTIGVAYML KLVHLVDDKI HARSTGPYSL VTQQPLGGKA QQGGQRFGEM
     EVWALEAFGA AYTLQELLTV KSDDMQGRNE ALNAIVKGKA IPRPGTPESF KVLMRELQSL
     GLDIAVHKVE TQADGSSLDV EVDLMADQLA RRTPPRPTYE SLSRESLDDD E