ID   RPOB_NOVAD              Reviewed;        1392 AA.
AC   Q2GCD7;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Saro_0024;
OS   Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG
OS   56034 / CIP 105152 / NBRC 16084 / F199).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Novosphingobium.
OX   NCBI_TaxID=279238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 /
RC   F199;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M.,
RA   Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N.,
RA   Fredrickson J., Balkwill D., Romine M.F., Richardson P.;
RT   "Complete sequence of Novosphingobium aromaticivorans DSM 12444.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000248; ABD24473.1; -; Genomic_DNA.
DR   RefSeq; WP_011443687.1; NC_007794.1.
DR   AlphaFoldDB; Q2GCD7; -.
DR   SMR; Q2GCD7; -.
DR   STRING; 279238.Saro_0024; -.
DR   EnsemblBacteria; ABD24473; ABD24473; Saro_0024.
DR   KEGG; nar:Saro_0024; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000009134; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1392
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000237308"
SQ   SEQUENCE   1392 AA;  154133 MW;  5FBB86EE13B66B42 CRC64;
     MATKATSSRT TGRPGVKKRI RKIFGDIHEV VQMPNLIEVQ RESYEQFLRS DPATGYVSGL
     EKTLRSVFPI QDFAGTASLD IKEKDGYVLE SPKYDVNECR QRGITYAAPM KVTLKLATFE
     VDAETETKSL IDIKEQDVYM GDIPLMTENG TFFINGTERV IVSQMHRSPG VLFDHDRGKT
     HSSGKYLFAA RVIPYRGSWL DFEFDAKDIV NVRIDRKRKL PVTALLHALG LGDEDILHHF
     YDTVTWARAE GGWKVPFVLE QWRGSKPAFD IVDAKSGEVI FPAGQKISPR AANKAAKDGL
     EALLIPTEEI FGRYAARDLI DESTGRIWIE AGDEVSPENL DLLDKAGIDN LDLLDIDHIS
     TGPWIRNTLK ADKAPDRDHA LADIYRVMRP GEPPTRETAE ALFDGLFFDG DRYDLSAVGR
     VKLNMRLGLD CPDTVTTLRT DDILAVVKEL VNLKDGKGEV DDIDNLGNRR VRSVGELLEN
     QYRVGLLRME RAVKERMSSV DVSTVMPNDL INAKPAVAAV REFFGSSQLS QFMDQTNPLS
     EVTHKRRVSA LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLSTFARVN
     KYGFIETPYR KVVDGKVTKD VIYLSAMEEQ KHTVAQASAE LTADGSFVEE LVSARQNGEF
     VMAPRDTVTL MDVSPKQLVS VAASLIPFLE NDDANRALMG SNMQRQAVPL IKADAPFVGT
     GMEETVARDS GAAISALRSG VVDQVDATRI VIRASGDIEP GQSGVDIYRL QKFERSNQST
     CINQRPLVKV GDLIEKGDII ADGPSTEFGE LALGRNVLVA FMPWNGYNYE DSILISERIV
     KEDVFTSIHI EEFEVMARDT KLGPEDITRD IPNVGEEALR NLDEAGIVYI GAEVHPGDIL
     VGKITPKGES PMTPEEKLLR AIFGEKASDV RDTSLRLPPG VAGTIVDVRV FNRHGIEIDD
     RTRAIQNEEI ERLAKDREDE RAILNRATFN RLKDMLLGQV AAAAPKGIKK GVEIDEQVLA
     DVERHEWWKF AVADDARQQQ LEAVKAQYDT AVKAIQEKFE DRKEKLERGD ELAPGVLKMV
     KVFVAVKRKL QPGDKMAGRH GNKGIISRIL PQEDMPFLED GTPVDFVLNP LGVPSRMNVG
     QIFETHLGWA ARGLGKRIGE ALDAWREANP NPEAGQPPEA VRELLKEIYG DNYAAEIDAR
     NDEQIIDFAQ SVRLGVPMGS PVFDGAVEKD VTDMLRLAGL DESGQVTLFD GRTGDAFDRK
     VTVGMKYVLK LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALQAYGAA
     YTLQEMLTVK SDDVVGRTKV YEAIVKGDDT FEAGIPESFN VLVKEMRSLG LNVELSSLDD
     TDDDDGLAEA AE