ID   RPOB_NYMAL              Reviewed;        1071 AA.
AC   Q6EW56;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Nymphaea alba (White water-lily) (Castalia alba).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Nymphaeales; Nymphaeaceae; Nymphaea.
OX   NCBI_TaxID=34301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15084683; DOI=10.1093/molbev/msh147;
RA   Goremykin V.V., Hirsch-Ernst K.I., Woelfl S., Hellwig F.H.;
RT   "The chloroplast genome of Nymphaea alba: whole-genome analyses and the
RT   problem of identifying the most basal angiosperm.";
RL   Mol. Biol. Evol. 21:1445-1454(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ627251; CAF28585.1; -; Genomic_DNA.
DR   RefSeq; YP_053147.1; NC_006050.1.
DR   AlphaFoldDB; Q6EW56; -.
DR   SMR; Q6EW56; -.
DR   GeneID; 2896155; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1071
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048033"
SQ   SEQUENCE   1071 AA;  120572 MW;  94701268EBDA8616 CRC64;
     MLRDGGDEGM FTIPGFSQIQ FEGFCRFIDQ GLMEELHQFP KIEDTDQEIE FQLFGESYQL
     VEPLIKERDA VYESITYSSE LYVPAGLIWR TGRNMQEQTV LLGNIPLMNS LGTSIVNGIY
     RIVINQILQS PGIYYSTGLD HNGISVYTGT IISDWGGRSE LEIDRKERIW ARVSRKQKIS
     ILVLSSAMGS SLREILDNVC YPEIFLSFPN EKEKKKIGSK ENAILEFYQK FACVGGDPVF
     SESLCKELQK KFFQQRCELG RIGRRNMNQR LNLDIPPNNT FLLPRDVLAA ADHLIGMKFG
     MGTLDDMNHL KNKRIRSVAD LLQDQFGLAL VRLENVVRGT ICGAIRHKLI PTPRNLVTST
     PLTTTYESFF GLHPLSQVLD RTNPLTQIVH GRKSSYLGPG GLTGRTASFR IRDIHPSHYG
     RICPIDTSEG INVGLIGSLA IHARVGDWGS IETPFYEISE RSKEEQMVYL SPSRDEYYMV
     AAGNSLALTR GIQEEEVGPA RYRQEFLTIA WEQIHLRNIY PFQYFSIGAS LIPFIEHNDA
     NRALMSSNMQ RQAVPLSQSE KCIVGTGLER QAALDSGGSA IAEREGKIIY TDAEKIVLSG
     NGDTISIPLV MYQRSNKNTW MHQKPQVHRG KYLKKGQILA DGAATVGGEL ALGKNVSVAY
     MPWEGYNSED AVLISERLVY DDIYTSFHIR KYEIQTHVTS QGPERITNEI PHLEPYLLRN
     LDRNGIVMLG SWVETGDVLV GKLTPQTAKE SSYAPEDRLL RAILGIQVST AKETCLKLPI
     GGRGRVIDVR WGQKKGGSIY NPEMIRVYIS QKRKIKVGDK VAGRHGNKGI ISKILPRQDM
     PYLQDGTPVD MVFNPLGVPS RMNVGQMFEC SLGLAGDLLG RHYRITPFDE RYEQEASRKL
     VFSELYEASK QTANPWVFEP EYPGKSRIFD GRTGDPFEQP VIIGKSYMLK LIHQVDDKIH
     GRSSGHYALV TQQPLRGRAK QGGQRVGEME VWALEGFGVA HILQEMLTYK SDHIRARQEV
     LGTTIVGGTI PNPEGAPESF RLLVRELRSL SLELNHFLVS EKNFQINRKE V