RPOB_OENEH
ID RPOB_OENEH Reviewed; 1072 AA.
AC Q9MTM5;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Oenothera elata subsp. hookeri (Hooker's evening primrose) (Oenothera
OS hookeri).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Oenothera.
OX NCBI_TaxID=85636;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Johansen;
RX PubMed=10852478; DOI=10.1007/pl00008686;
RA Hupfer H., Swiatek M., Hornung S., Herrmann R.G., Maier R.M., Chiu W.-L.,
RA Sears B.;
RT "Complete nucleotide sequence of the Oenothera elata plastid chromosome,
RT representing plastome I of the five distinguishable Euoenothera
RT plastomes.";
RL Mol. Gen. Genet. 263:581-585(2000).
RN [2]
RP SEQUENCE REVISION TO 699 AND 734-735.
RX PubMed=18299283; DOI=10.1093/nar/gkn081;
RA Greiner S., Wang X., Rauwolf U., Silber M.V., Mayer K., Meurer J.,
RA Haberer G., Herrmann R.G.;
RT "The complete nucleotide sequences of the five genetically distinct plastid
RT genomes of Oenothera, subsection Oenothera: I. Sequence evaluation and
RT plastome evolution.";
RL Nucleic Acids Res. 36:2366-2378(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AJ271079; CAB67151.2; -; Genomic_DNA.
DR RefSeq; NP_084686.2; NC_002693.2.
DR AlphaFoldDB; Q9MTM5; -.
DR SMR; Q9MTM5; -.
DR GeneID; 802745; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1072
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048035"
SQ SEQUENCE 1072 AA; 121112 MW; 80F4E6D1AC2543B5 CRC64;
MLGDGNGGMC TIPGFNQIQF EGFCRFIDQG LTEELSKFPK IEDDTDQEIE FQLFVETYHL
VEPLIKERDA VYELLTYSSE LYISAGLIWK SSRDMQKQTI FIGNIPLMNS LGTSIVNGIY
RIVINQILQS PGIYYRSELD HNGISVYTGT IISDWGGRLE LEIDRKARIW ARVSRKQKIS
ILVLSSAMGS NLREILENVC YPEMFLSFLN EKEKKTIGSK ENAILEFYQQ FSCVGGDPVF
SESLCKELHT KFFQQRCELG RIGRQNMNRR LNLDIPQHNT FLLPRDILAA ADHLIGMKFG
MGTLDDTNHL KNKRIRSVAD LLQDQFGLAL VRLESVVRRT ICGAIRHKLI PTPHNLVTST
PLTTTYESFF GLHPLSQVLD RTNPLTQIVH GRKLSYLGPG GLTARTASFR MRDIHPSYYG
RICPIDTSEG INVGLIGSLA IHARIGRGGS LESPFYEISE RSNKGRMLYF SPSRDEYYMV
AAGNSLALNR GIQEEQVVPA RYRQEFLTLA WDQVHLRSIF PFQYFSIGAS LIPFIEHNDA
NRALMSSNMQ RQAVPLSQSE RCIVGTGLER QASLDSGVTA IAEREGKIIY TNSDKIILSG
SGDTLSIPLV MYQRSNKNTW MHQKPQVPRG KCIKKGQILA DGAATVGGEL ALGKNVLVAY
MPWEGYNFED AVLISERLVY EDIYTSFHIR KYEMQTHVTS QGPERITNEI PHLEAHLLRN
LDKNGIVMMG SWVEAGEILV GKLTPQMAEE ESSYAPEERL LRAILGIQIS TSKETCLKLP
IGGRGRVIDV RWIQKRGGSS YNPETIRVYI LQKREIKVGD KVAGRHGNKG IISKILPRQD
MPYLQDGRPV DMVFNPLGVP SRMNVGQIFE CSLGFAGDLL ERHYRIAPFD ERYEQEASRK
LVFSELYEAS KQTANPWVFE PEYPGKSRLF DGRTGDPFEQ PVIIGKPYIL KLIHQVDDKI
HGRSSGHYAR VTQQPLRGRA KQGGQRVGEM EVWALEGFGV AHLLQEMLTY KSDHIRARQG
VLGTTILGGT IPKPEDAPES FRLLVRELRS LALELNHFLV SEKNFQINRK EA
