RPOB_ORITB
ID RPOB_ORITB Reviewed; 1377 AA.
AC A5CC93;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=OTBS_0208;
OS Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Orientia.
OX NCBI_TaxID=357244;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Boryong;
RX PubMed=17483455; DOI=10.1073/pnas.0611553104;
RA Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y.,
RA Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S.,
RA Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.;
RT "The Orientia tsutsugamushi genome reveals massive proliferation of
RT conjugative type IV secretion system and host-cell interaction genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM494475; CAM79274.1; -; Genomic_DNA.
DR RefSeq; WP_011944332.1; NC_009488.1.
DR AlphaFoldDB; A5CC93; -.
DR SMR; A5CC93; -.
DR EnsemblBacteria; CAM79274; CAM79274; OTBS_0208.
DR KEGG; ots:OTBS_0208; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001565; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300362"
SQ SEQUENCE 1377 AA; 153662 MW; EDAEE74270E7EC10 CRC64;
MQYLLSKKRI RKNFGKINLV SSIPNLIEVQ KNSYNKEFLQ LGVPVDCREN KGLQGVLNSI
FPIHDLDGSA ILEFVRYDFD EPKYDVEECI QRGLSYTAAL KITLSLIVLD DSDKSDPKEI
KGIKEQAVYF GDIPLMTANG TFVINGTERV VVSQMHRSPG VFFDHDDGKT HSTGKLIYSA
RVIPYRGSWL DFEFDAKDLL YFRIDRKRKL YVTTLLMALG MSSHDILNFY YESATYKKYK
KSENGWIVDF LPELVSTKHL AYDLIDAQTG KVLLNAGQKI TFRIAKMLAD KGVKQILVKN
DSLVGKLLAE DLVNNQTGEV LLGVGEEITN DILTIVNDLQ ITTVKVLAVG GQCGPYVRNT
LFLDKNKDQK SSLVEIFRVL KPGEMATTAA SRGLLDSLLF DNNRYDLSEV GRIKINSRLN
LDIDLKNTCI TVEDIKKIIK LLVDIKDGKA LVDDIDHLGN RRVRSVGELV ENQFRTGLVR
IAKFIVERIG SVEIDAVVPN DLVNAKLLTS VIKEFFGTSQ LSQFMDQTNA LSQITHIRRL
SALGPGGLNR DRAALEVRDV HPTHYGRICP IETPEGQNIG LINSLAIFAK INRYGFIESP
YRRVINRRVT DEVVYLSALE EGRYKIAQAD TSLCTNDCLI DGLVSCRYDG NFVMVLAQEV
DFIDLTPMQV VSVAASMIPF LENDDANRAL MGANMQRQAV PLLKSQAPLV GTGIESVVAH
DSGTVVAALH DGIVEQVDST RIVVKTSVKK DAGAPGVDIY NLKKFQRSNY NTCINQKVLV
NVGDIVKKGD VIADGVATNK GEIALGRNVL VAFIAWNGYT FEDSILVSER IVKEDIYTSI
HIEELELVAR DTRLGPEEIT RDIPNVNDES LRHLDEVGIV NIGAKVRTGD ILVGKVTPKS
ESPVTPEEKL LRAIFGEKAS EVKDSSLYVP PGITGTVIDV RVFSRRGIDK DERTLAIEKQ
KIRKLVKDYE DELAVIQHFT IVRVKELLVG QVAACSLDSI IIGDKLDEAM LNKLTNEQLL
KLKADNSSIM DEISEIKCHY ETTRAQLTSQ LNSKIEKIQC GDDLPQGALR VVKVFIAIKH
RLQPGDKMAG RHGNKGVVSK VVPEEDMPFL EDGTVVDIVL NPLGLPARMN VGQILETHLG
WAGVNLGRKI GKMVDDYYSG NNQCTDEIRK FVKKIYANNS IADTIDQVND QQLIEICQEL
RQGIYFSTPV FDGAKIADIK QMLELADVDK SGQVRLIDGR TGEYFDRRCT VGYQYLLKLH
HLVDEKIHAR SIGPYSLVTQ QPLGGKSHFG GQRFGEMECW ALEAYGAAYT LQELLTTKSD
SVEGRIKMYQ AIIRGNNNFV SGVPESLNVM IKELRSLCLN IQLEENDEED ENEDENY
