RPOB_ORYNI
ID RPOB_ORYNI Reviewed; 1075 AA.
AC Q6ENI4;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Oryza nivara (Indian wild rice) (Oryza sativa f. spontanea).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=4536;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. SL10 {ECO:0000312|Proteomes:UP000006591};
RX PubMed=15556301; DOI=10.1016/j.gene.2004.06.008;
RA Masood M.S., Nishikawa T., Fukuoka S., Njenga P.K., Tsudzuki T.,
RA Kadowaki K.;
RT "The complete nucleotide sequence of wild rice (Oryza nivara) chloroplast
RT genome: first genome wide comparative sequence analysis of wild and
RT cultivated rice.";
RL Gene 340:133-139(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AP006728; BAD26768.1; -; Genomic_DNA.
DR RefSeq; YP_052739.1; NC_005973.1.
DR AlphaFoldDB; Q6ENI4; -.
DR SMR; Q6ENI4; -.
DR GeneID; 2885962; -.
DR Proteomes; UP000006591; Chloroplast.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IC:Gramene.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IEA:EnsemblPlants.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0035196; P:miRNA processing; IEA:EnsemblPlants.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..1075
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048036"
SQ SEQUENCE 1075 AA; 121642 MW; 1F55287D707209BB CRC64;
MLRNGNEGMS TIPGFSQIQF EGFCRFINQG LAEELEKFPT IKDPDHEISF QLFAKGYQLL
EPSIKERDAV YESLTYSSEL YVSARLIFGF DVQKQTISIG NIPIMNSLGT FIINGIYRIV
INQILLSPGI YYRSELDHKG ISIYTGTIIS DWGGRSELAI DKKERIWARV SRKQKISILV
LSSAMGSNLK EILDNVSYPE IFLSFPNAKE KKRIESKEKA ILEFYQQFAC VGGDLVFSES
LCEELQKKFF QQKCELGRIG RRNMNRRLNL DIPQNSTFLL PRDVLAATDH LIGMKFETGI
LDDDDMNHLK NKRIRSVADL LQDQFGLALG RLQHAVQKTI RRVFIRQSKP TPQTLVTPTS
TSILLITTYE TFFGTYPLSQ VFDQTNPLTQ TVHGRKVSCL GPGGLTGRTA SFRSRDIHPS
HYGRICPIDT SEGINVGLTG SLAIHARIDH WWGSVESPFY EISEKAKKKK ERQVVYLSPN
RDEYYMIAAG NSLSLNRGIQ EEQVVPARYR QEFLTIAWEQ IHVRSIFPFQ YFSIGGSLIP
FIEHNDANRA LMSSNMQRQA VPLSRSEKCI VGTGLERQTA LDSRVSVIAE REGKIISTNS
HKILLSSSGK TISIPLVTHR RSNKNTCMHQ KPRVPRGKSI KKGQILAEGA ATVGGELALG
KNVLVAYMPW EGYNFEDAVL ISERLVYEDI YTSFHIRKYE IQTDTTSQGS AEKITKEIPH
LEEHLLRNLD RNGVVKLGSW VETGDILVGK LTPQIASESS YIAEAGLLRA IFGLEVSTSK
ETSLKLPIGG RGRVIDVKWI QRDPLDIMVR VYILQKREIK VGDKVAGRHG NKGIISKILP
RQDMPYLQDG TPVDMVFNPL GVPSRMNVGQ IFESSLGLAG DLLKKHYRIA PFDERYEQEA
SRKLVFSELY EASKQTKNPW VFEPEYPGKS RIFDGRTGDP FEQPVLIGKS YILKLIHQVD
EKIHGRSTGP YSLVTQQPVR GRAKQGGQRI GEMEVWALEG FGVAHILQEI LTYKSDHLIA
RQEILNATIW GKRVPNHEDP PESFRVLVRE LRSLALELNH FLVSQKNFQV NREEV
