ID   RPOB_OSTTA              Reviewed;        1088 AA.
AC   Q0P3M6;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=OtCpg00260;
OS   Ostreococcus tauri.
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC   Bathycoccaceae; Ostreococcus.
OX   NCBI_TaxID=70448;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OTTH0595;
RX   PubMed=17251180; DOI=10.1093/molbev/msm012;
RA   Robbens S., Derelle E., Ferraz C., Wuyts J., Moreau H., Van de Peer Y.;
RT   "The complete chloroplast and mitochondrial DNA sequence of Ostreococcus
RT   tauri: organelle genomes of the smallest eukaryote are examples of
RT   compaction.";
RL   Mol. Biol. Evol. 24:956-968(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CR954199; CAL36351.1; -; Genomic_DNA.
DR   RefSeq; YP_717229.1; NC_008289.1.
DR   AlphaFoldDB; Q0P3M6; -.
DR   SMR; Q0P3M6; -.
DR   STRING; 70448.Q0P3M6; -.
DR   PRIDE; Q0P3M6; -.
DR   GeneID; 4238798; -.
DR   KEGG; ota:OstapCp26; -.
DR   eggNOG; KOG0214; Eukaryota.
DR   InParanoid; Q0P3M6; -.
DR   OrthoDB; 7046at2759; -.
DR   Proteomes; UP000009170; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1088
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276593"
SQ   SEQUENCE   1088 AA;  122797 MW;  7B81B60B9DE4C4D7 CRC64;
     MTLFRQNSAL PNFLDSQRDS FRYFLETGIR EELDFFSPIV GQSLGSSSKR PTDRFISVSF
     HSKDFYFKKP HYTPQEAVQK LGTYKSSLIV PVHVYSKYLN LNATFPVAFC DLPLMTEHGT
     FILNGSPRVI VHQIVRCPGV YLKPQFDKQG NRTHLVSFLS AYGSWLRFET DKKGVVFAHI
     DNLRKVPVTV FLQALGFSMD TIVGALKYPE ALDPTLKEVD WKLTTDEAIL LLMSRLFPNR
     PATVLRGRKF LFNQFFNPRR YSLSDVGRKR VNQKFRMRSK TKHLTLTPQD ALAALDYLLR
     CENGETEFLD DIDHLKNRRA RLAGELIQTQ FRLGLNRLER VIYNRISDEN ILRKTPGALG
     SLNSLIRTQV LASVFQEFFG SNQLSQFMDQ TNPLAEITHK RRLSSLGPGG LNRDRAGLIV
     RGIHPSYYGR ICPIETPEGK NAGLVGSIAT FTQINKNGFL ESPYYKLISE SNTPDRNGFF
     LLSAFYEEDT VVAQGDVDLS NFRIPTRNKS QFTENTVQEI NFLGLCPIQF MSIATSLIPF
     LEHDDANRAL MGSNMQRQAV SLLRSERPFV GTGLEAHVTR DIGATIVAKQ NSYISYVDAQ
     RIDYFTPVIG DTNLIDYQNL TAEDVFASNQ FKHNTIWLTS YQRSNQDTCL NHKPLVEANT
     WVEAGDCLAD NAATAKGELA LGRNILIGYM PWEGYNFEDA VLVSERLVYD DVFTSIHISR
     YEVSTARLRE GQEYFTNQVD RNQYLDEFGV VKIGTWVEAG DVLVGKISPQ PDSDNDPESR
     LLRAIFGGVA RNTKTTSYCL SSGVSGRILD VRCEFKRQTK NIEDESIEST GSVYVYLVEK
     RRLQVGDKVA GRHGNKGIVS NILPRVDMPY LQSGKALDMV LNPLGVPSRM NVGQIFECLL
     GLAANTLKQN FKVLPFDEMH GAEVSRGFVY HYLYKSRLLT QQKWLFKPNS PGKSIVFDGR
     TGLNFDQPVT VGYPYILKLV HLVDDKIHAR STGPYSLVTQ QPLGGRSKKG GQRLGEMEVW
     ALEGFGAAYV LQELLTIKSD DMIGRNRAFM SMIRGTLLPK SGIPESFKVL VSELRGLCLD
     MSIARINF