RPOB_PAEAT
ID RPOB_PAEAT Reviewed; 1173 AA.
AC A1R8V4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=AAur_2957;
OS Paenarthrobacter aurescens (strain TC1).
OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Paenarthrobacter.
OX NCBI_TaxID=290340;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TC1;
RX PubMed=17194220; DOI=10.1371/journal.pgen.0020214;
RA Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B.,
RA Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V.,
RA Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.;
RT "Secrets of soil survival revealed by the genome sequence of Arthrobacter
RT aurescens TC1.";
RL PLoS Genet. 2:2094-2106(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000474; ABM06631.1; -; Genomic_DNA.
DR AlphaFoldDB; A1R8V4; -.
DR SMR; A1R8V4; -.
DR STRING; 290340.AAur_2957; -.
DR EnsemblBacteria; ABM06631; ABM06631; AAur_2957.
DR KEGG; aau:AAur_2957; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_11; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000000637; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1173
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300277"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1173 AA; 128956 MW; 2E16A007610E3ADC CRC64;
MEGSLLVASS TSNNETANTA STDGATRRLS FAKIHEPLDV PNLLALQTDS FDWLVGNERW
QARVAKAVEE GDLSVATTSG LADIFEEISP IEDFQGTMSL SFSEPEFADP KYTMAECKDR
DATYSAPLYV KAEFMNNNTG EIKQQTVFMG DFPLMTEKGT FVVNGTERVV VSQLVRSPGA
YFERTADKTS DKDIFTAKII PSRGAWFELE IDKRDQVGVR LDRKRKQSVT VLLKALGWTE
GQILEEFGQY DSMRATLEKD ATETREDALL DIYRKLRPGE PPTVEAAQSL LDNLYFNAKR
YDLAKVGRYK INRKLGIDRS LGDKEASVLH VEDIVAMIKF LVALHAGEKT LMGKRDGEDH
ELRVDVDDID HFGNRRIRAV GELIENQVRT GLSRMERVVR ERMTTQDVEA ITPQTLINIR
PVVAAIKEFF GTSQLSQFMD QNNPLSGLTH KRRLSALGPG GLSRDRAGME VRDVHPSHYG
RMCPIETPEG PNIGLIGSLA SYGRINPFGF IETPYRLVSE GVVSDEVQYL TADDEAEVLI
AQANAPLDAD KKFSEETVLV RARGGGGEPV LVPAADVQFM DVSPRQMVSV ATALIPFLEH
DDANRALMGA NMQRQAVPLV RSEAPFVGTG MERAAAVDAG DVVIAKKAGV VTEVSAELVV
MINDDGTETN YRINKFARSN QGNCYNHRVL VNEGQRLEVG GIIADGPATD QGELALGKNL
LVAFMSWEGH NFEDAIILSQ RIVAEDVLSS IHIEEHEIDA RDTKLGAEEI TRDIPNVSEE
VLAGLDERGI IHIGAEVEAG DILVGKVTPK GETELTPEER LLRAIFGEKS REVRDTSLKV
PHGESGTVIG VRVFDRDNDD ELPPGVNQLV RVYVAAKRKI TDGDKLAGRH GNKGVISKIL
PIEDMPFLAD GTPVDIVLNP LGVPGRMNVG QVLETHLGWV AKTGWKIEGE PEWVKNLPNL
PRETGQTTVA TPVFDGAREE EITGLLDSTN VTRDGDRLID SSGKTRLFDG RSGEPFPDPI
SVGYMYILKL HHLVDDKIHA RSTGPYSMIT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY
TLQELLTIKS DDIHGRVKVY EAIVKGENIP EPGVPESFKV LIKEMQSLCL NVEVLSTDGT
TIEMRDSDDA VFTAAEELGI DLSRAEPSSV EEV
