ID   RPOB_PANGI              Reviewed;        1071 AA.
AC   Q68S14;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; ORFNames=PSC0250;
OS   Panax ginseng (Korean ginseng).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Araliaceae; Panax.
OX   NCBI_TaxID=4054;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15500250; DOI=10.1093/dnares/11.4.247;
RA   Kim K.-J., Lee H.-L.;
RT   "Complete chloroplast genome sequence from Korea ginseng (Panax schinseng
RT   Nees) and comparative analysis of sequence evolution among 17 vascular
RT   plants.";
RL   DNA Res. 11:247-261(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AY582139; AAT98501.1; -; Genomic_DNA.
DR   RefSeq; YP_086958.1; NC_006290.1.
DR   AlphaFoldDB; Q68S14; -.
DR   SMR; Q68S14; -.
DR   GeneID; 3021561; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1071
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048038"
SQ   SEQUENCE   1071 AA;  120830 MW;  84A1BEBD3A6BB42B CRC64;
     MLRDGKNEGM STIPGFNQIQ FEGFCRFIDQ GLTEELYKFP KIEDTDQEIE FQLFVETYQL
     VEPLIKERDA VYESLTYSSE LYVSAGLIWK TGRDMQEQTI FFGNIPLMNS LGTSIVNGIY
     RIVINQVLQS PGIYYRSELD HNGISVYTGT IISDWGGRSE LEIDRKARIW ARVSRKQKIS
     ILVLSSAMGS NLREILENVC YPEIFLSFLT DKEKKKIGSK ENAILEFYQQ FACVGGDPVF
     SESLCKELQK KFFQQRCELG RIGRRNMNRR LNLDISQNNT FLLPRDILAA ADHLIGMKFG
     MGTLDDMNHL KNKRIRSVAD LLQDQFGLAL VRLENVVRGT ICGALRHKLI PTPQNLVTST
     PLTTTYESFF GLHPLSQVLD RTNPLTQIVH GRKLSYLGPG GLTGRTASFR IRDIHPSHYG
     RICPIDTSEG INVGLIGSLA IHARIGRWGS LESPFYEISE RSKGARMLYL SPGKDEYYMV
     AAGNSLALNQ GIQEEQVVPA RYRQEFLTIA WEQVHLRSIF SFQYFSIGAS LIPFIEHNDA
     NRALMSSNMQ RQAVPLSRSE KCIVGTGLER QAAIDSGALA IAEHEGKIIY TDTDKILLSG
     NGNTLSIPLV IYQRSNKNTC MHQKPQVQRG KCIKKGQILA HGAATVGGEL ALGKNVLVAY
     MPWEGYNFED AVLISERLVY EDIYTSFHIR KYEIKTHVTS QGPERVTNEI PHLEAHLLRN
     LDKNGIVMLG SWVETGDILV GKLTPQMVKE SSYAPEDRLL RAILGIQVST SKETCLKLPI
     GGRGRVIDVR WIQKRGGSSY NPEMIRVYIS QKREIKVGDK VAGRHGNKGI ISKILPRQDM
     PYLQDGRPVD MVFNPLGVPP RMNVGQIFEC SLGLAGGLLD RHYRIAPFDE RYEQEASRKL
     VFSELYEAGK QTANPWVFEP EYPGKSRIFD GRTGDPFEQP VIIGKPYILK LIHQVDDKIH
     GRSSGHYALV TQQPLRGRAK QGGQRVGEME VWALEGFGVA HILQEMLTYK SDHIRARQEV
     LGTTIIGGTI PNPQDAPESF RLLVRELRSL ALELNHFFVS EKNFQINRKE A