ID   ATRGC_MALDO             Reviewed;         459 AA.
AC   A0A498KE69; V9P9M2;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Alcohol acyl transferase 1 allele RGc {ECO:0000303|PubMed:24661745};
DE            Short=AAT1-RGc {ECO:0000303|PubMed:24661745};
DE            EC=2.3.1.- {ECO:0000250|UniProtKB:Q64FJ6};
GN   Name=AAT1RGC {ECO:0000303|PubMed:24661745};
GN   ORFNames=DVH24_017673 {ECO:0000312|EMBL:RXI05631.1};
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. HFTH1; TISSUE=Leaf;
RA   Hu J.;
RT   "A high-quality apple genome assembly.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-459, TISSUE SPECIFICITY,
RP   DEVELOPMENTAL STAGE, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Royal Gala;
RX   PubMed=24661745; DOI=10.1111/tpj.12518;
RA   Souleyre E.J.F., Chagne D., Chen X., Tomes S., Turner R.M., Wang M.Y.,
RA   Maddumage R., Hunt M.B., Winz R.A., Wiedow C., Hamiaux C., Gardiner S.E.,
RA   Rowan D.D., Atkinson R.G.;
RT   "The AAT1 locus is critical for the biosynthesis of esters contributing to
RT   'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.";
RL   Plant J. 78:903-915(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC       ripe apple fruit flavor (By similarity). Alcohol acyl transferase that
CC       can use a wide range of alcohols as substrate to produce esters (By
CC       similarity). {ECO:0000250|UniProtKB:Q64FJ6}.
CC   -!- TISSUE SPECIFICITY: Expressed at very low levels in the skin of ripe
CC       fruit. {ECO:0000269|PubMed:24661745}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates progressively at very low levels
CC       during fruit development. {ECO:0000269|PubMed:24661745}.
CC   -!- MISCELLANEOUS: The fruit of cv. Royal Gala exhibits a high ester
CC       accumulation, whereas the cv. Granny Smith contains low ester levels;
CC       this influences strongly the ripe apple fruit aroma.
CC       {ECO:0000305|PubMed:24661745}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; RDQH01000328; RXI05631.1; -; Genomic_DNA.
DR   EMBL; KC291131; AGW30202.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A498KE69; -.
DR   SMR; A0A498KE69; -.
DR   STRING; 3750.XP_008368021.1; -.
DR   Proteomes; UP000290289; Chromosome 2.
DR   GO; GO:0016746; F:acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006066; P:alcohol metabolic process; ISS:UniProtKB.
DR   GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase.
FT   CHAIN           1..459
FT                   /note="Alcohol acyl transferase 1 allele RGc"
FT                   /id="PRO_0000451710"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   ACT_SITE        385
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   VARIANT         307
FT                   /note="R -> G (in strain: cv. Royal Gala)"
FT                   /evidence="ECO:0000305"
FT   VARIANT         454..459
FT                   /note="RIMSMM -> SQ (in strain: cv. Royal Gala)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   459 AA;  51266 MW;  FA817A767DA9925B CRC64;
     MMPFSVLQVK RLQLELITPA KPTLQETKFL SDIDDQEGLR FQVPVIMCYK DNPSLNKNCN
     PVKVIREALS RALVYYYPLA GRLKEGPNRK LMVDCNGEGI LFVEASADVT LEQLGDKILP
     PCPLLEEFLF NFPGSDGIIG CPLLLVQVTC LTCGGFILAL RVNHTMCDAP GLLLFLTAIA
     EMARGAHAPS ILPVWERELL FSRDPPRITC VHHEYEDVID HSDGSYASSN QSNMVQRSFY
     FGAKEMRVLR KQIPPHVIST CSTFDLITAC LSKCRTLALK INPKQAVRVS CVVNARGKHH
     NVRLPLRYYG NAFAFPTAVS KAEPLCKNPL GYALELVKKA KATMNEEYLR SVADLLVLRG
     RPQYSSTGSY LIVSDNTRAG FGDVNFGWGQ PVFAGPAKAL DLISFYVQHK NNTEDGILVP
     MCLPSSAMER FQQELERITQ EPKEDICNNL RSTRIMSMM