RPOB_PELHO
ID RPOB_PELHO Reviewed; 1079 AA.
AC Q06FW9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Pelargonium hortorum (Common geranium) (Pelargonium inquinans x Pelargonium
OS zonale).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Geraniales; Geraniaceae; Pelargonium.
OX NCBI_TaxID=4031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Ringo White;
RX PubMed=16916942; DOI=10.1093/molbev/msl089;
RA Chumley T.W., Palmer J.D., Mower J.P., Fourcade H.M., Calie P.J.,
RA Boore J.L., Jansen R.K.;
RT "The complete chloroplast genome sequence of Pelargonium x hortorum:
RT organization and evolution of the largest and most highly rearranged
RT chloroplast genome of land plants.";
RL Mol. Biol. Evol. 23:2175-2190(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; DQ897681; ABI17253.1; -; Genomic_DNA.
DR RefSeq; YP_784062.1; NC_008454.1.
DR AlphaFoldDB; Q06FW9; -.
DR SMR; Q06FW9; -.
DR GeneID; 4362772; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1079
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000276594"
FT REGION 963..982
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1079 AA; 122558 MW; 40694F352E3AB945 CRC64;
MLGDRNEGMA TIPALNQIQF EGFCRFLDQG LIEELSQFPK GNSFLEYFRD EEIDFQLFSK
TYHLVKPLIK ERDARYESLT YSSKLYVSAG FLWKDKRPQK KTICIGNLPL MNSVGTFIVN
GISRVVINQI LQSPGIYYGS ELDYQGFTVY IATIISDWGG RLKLEIDKKE RIWARVSRKQ
KISILVLSSA MGSNLKEILE NACYPEIFLA LNLKKECGFK EKKYAILELY AKYKDFTEYK
DSLDFDFLCE TLRKTFFSHK CELGRVGRQN INRRLNLDIS HKNTFLLPQD ILAATNHLIG
MKFGMGTLDD MNHLKNKRIR SVADLLQDQF GLALNRLEDA VCDTIGRAIA KDKIPTLRHL
ATSTPLTTTY ESFFKLHPLS HVFDETNPLT HIVHGRKWSF LGPGGLTERT ARFRVRDIHP
SNYGRICPID TSEGIKVGLI GYLATHARIG DCGSLESPFY EMSEKSKRVR MLSLSSRKEE
YYMIGTGNSL ALNQGIQEEQ IIPARYRQEF LTIAWEEVHF RSVFPFQYFS IGVSLIPFLE
HNDATRALMS SNMQRQAVPL SRSEKCIVGT GFEGQVALDS GIPLITEHAG KIISTHTDKI
LLSVNGETLS SPLVLFQRSN KNTWIHQTPQ VSRGNCTKKG QILADGAATV GGELALGKNV
LVGYMPWEGY NFEDAVLISE RLVYEDIYTS FHIRKCEIKI FMTMKGPEKI TNQIPHIDPH
LLRNLDKNGV VILGSWVEAG DILVGKLTPE LVELSPEKRL IEALFDAPIG TTRQSCLKLP
IWGRGRVIDV RWIHKGSASR SNPERIRVYI LQKRKIKVGD KVAGRHGNKG IVSKILPIQD
MPYLQDGRPL DMVFNPLGVP SRMNVGQIFE CSLGLAGGLL DRHYRIAPFD ERYEEEASRK
LVFSELYEAS KQTGNPWVFE PEYPGKSIIF DGRTGDPFEQ PVIVGQSYIL KLIHQVDDKI
HGRSTGPYSR VTQQPVKGRA RRGGQRVGEM EVWALQGLGV AHILEEILTY KSDHLTTRNQ
IFGTLLLGGA LPKPEPEDRP ESFRLLVREL RSLSLELNYF LVSKNKKNFN FWKMNRVEV
