RPOB_PHAVU
ID RPOB_PHAVU Reviewed; 1070 AA.
AC A4GGA5; A8W812;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Negro Jamapa;
RX PubMed=17623083; DOI=10.1186/1471-2164-8-228;
RA Guo X., Castillo-Ramirez S., Gonzalez V., Bustos P.,
RA Fernandez-Vazquez J.L., Santamaria R.I., Arellano J., Cevallos M.A.,
RA Davila G.;
RT "Rapid evolutionary change of common bean (Phaseolus vulgaris L) plastome,
RT and the genomic diversification of legume chloroplasts.";
RL BMC Genomics 8:228-228(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Moore M.J., Triplett E.W., Broughton W.J., Soltis P.S., Soltis D.E.;
RT "Complete nucleotide sequence of the plastid genome of the common bean,
RT Phaseolus vulgaris.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; DQ886273; ABH88086.1; -; Genomic_DNA.
DR EMBL; EU196765; ABW22782.1; -; Genomic_DNA.
DR RefSeq; YP_001122806.1; NC_009259.1.
DR AlphaFoldDB; A4GGA5; -.
DR SMR; A4GGA5; -.
DR STRING; 3885.XP_007141910.1; -.
DR GeneID; 4961795; -.
DR KEGG; pvu:PhvuCp22; -.
DR eggNOG; KOG0214; Eukaryota.
DR PhylomeDB; A4GGA5; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1070
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300451"
SQ SEQUENCE 1070 AA; 120609 MW; 593EB4C2488EB138 CRC64;
MLGSGNEEMS TLPGLNQIQL EGFCRFIDRG LTEGLFKFPK IEDTDQEIEF QLFAETYQLL
EPLINEKDAV YESLTYSAEL YVSTGLIWKS SRDIKKQTIF VGNIPLMNSL GTSIVNGIYR
IVINQILQSP GIYYRSELDP NGISVYTGTI ISDWGGRLEL EIDKKARIWA RVSRKQKISI
LILLSAMGSN LSEILENVCY PEIFVSFLND KDKKKIGSKE NAILEFYRQF ACVGGDPVFS
ESLWKELQKK FFQQRCELGR IGRRNMNQKL NLDIPQNNTF LLPRDILTAA SHLIGMKFGM
ALLDDINHLK NKRIRSVADL LQDQFGLALV RLENMVRGTI CGAIRHKLIP TPQNLVTSTP
LTTTYESFFG LHPLSQVLDQ TNPLTEIVHG RKLSYLGPGG LTGRTASFRI RDIHPSHYGR
ICPIDTSEGI NVGLIGSLAI HARIGIWGAI ESPFFEISER SKRIRMLYLS PSIDEYYMVA
TGNSLALTRD IQEEQIVPAR YRQEFLTIAW EQVHLRSIYP FQYFSIGASL IPFIEHNDAN
RALMSSNMQR QAVPLSQSEK CIVGTGLECQ VALDSGVSAI ADHEGNIVYT DTDRIFLFVN
GDTLSIPLTI YQRSNKNTCM HQKPQVHRGK FIKKGQILAD GAATVGGELA LGKNVLVAYM
PWEGYNSEDA VLISERLVYE DIFTSFHIRK YEIQTHMTSY GSERITNKIP HLEANLLRNL
DKNGIVILGS WVETGDVLVG KLTPQMAKES SYSPEDRLLR AILGIQVSTS KETCLKLPIG
GRGRVIDVRW IQKKGGSSYN PETIRISILQ KREIKVGDKV AGRHGNKGIV SKILSRQDMP
YLQDGEPVDM VFNPLGVPSR MNVGQIFECS LGLSGGMLDK HYRITPFDER YEQEASRKLV
FSELYEASKK TSNPWIFEPE YPGKSKIFDG RTGNSFKQPA IMGKTYILKL IHQVDDKIHG
RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIKTRQEVL
GTTIIGGTIP KPTDAPESFR LLVRELRSLA MELNHFLVSE KNFRIHRKEA
