RPOB_PHEZH
ID RPOB_PHEZH Reviewed; 1356 AA.
AC B4R8K5;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=PHZ_c1218;
OS Phenylobacterium zucineum (strain HLK1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Phenylobacterium.
OX NCBI_TaxID=450851;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HLK1;
RX PubMed=18700039; DOI=10.1186/1471-2164-9-386;
RA Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.;
RT "Complete genome of Phenylobacterium zucineum - a novel facultative
RT intracellular bacterium isolated from human erythroleukemia cell line
RT K562.";
RL BMC Genomics 9:386-386(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000747; ACG77632.1; -; Genomic_DNA.
DR RefSeq; WP_012521777.1; NC_011144.1.
DR AlphaFoldDB; B4R8K5; -.
DR SMR; B4R8K5; -.
DR STRING; 450851.PHZ_c1218; -.
DR PRIDE; B4R8K5; -.
DR EnsemblBacteria; ACG77632; ACG77632; PHZ_c1218.
DR KEGG; pzu:PHZ_c1218; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001868; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1356
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141716"
SQ SEQUENCE 1356 AA; 151282 MW; 4EDABC4BCFEBA45B CRC64;
MAQSFTGKKR IRKSFGRIPE AVQMPNLIEV QRSSYEQFLQ REVRAGERRD EGIEAVFKSV
FPIKDFNERA VLEYVSYEFE EPKYDVEECV QRDMTYAAPL KVKLRLIVFE TDEETGARSV
KDIKEQDVYM GDIPLMTEKG TFIVNGTQRV IVSQMHRSPG VFFDHDKGKT HASGKLLFAA
RVIPYRGSWL DFEFDAKDIV YVRIDRRRKL PATTFLMALG MDGEEILSTF YETVPYEKKG
DGWATPYKPE RWRGVKPEFP LIDADTGEEI APAGQKISAR NAKKFADNGL KTLLLAPEAL
TGRYLAKDLV NFETGEIYAE AGDELDPTLL AALEEQGFTT LDVLDIDEVT VGAYIRNTLR
VDKNTAREDA LFDIYRVMRP GEPPTVEAAE AMFKSLFFDS ERYDLSSVGR VKMNMRLELD
CPDDVRVIRK EDVIAVLLTL VGLRDGRGEI DDIDNLGNRR VRSVGELLEN QYRVGLLRME
RAIKERMSSV DIDTVMPHDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EITHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATHAVVN KYGFIESPYR
RIRDGKTTDE VVYMSAMEEA KHVIAQANIK LENGEIVEDL VPGRINGEPS LLPKADVDLM
DVSPKQVVSV AASLIPFLEN DDANRALMGS NMQKQAVPLI QSDAPLVGTG MESIVAVDSG
AVVVARRTGV VEQIDGTRIV VRATEETDPS KPGVDIYRLQ KFQRSNTSTC INQRPLVRVG
DKINAGDVIA DGPSTELGEL ALGRNALVAF MPWNGYNFED SILISERIVR DDVFTSIHIE
EFEVMARDTK LGPEEITRDI PNVGEEALRN LDEAGIVAIG AEVQPGDILV GKVTPKGESP
MTPEEKLLRA IFGEKASDVR DTSLRLPPGV SGTIVEVRVF NRHGVDKDER ALAIERAEID
RLGKDRDDEF AILNRNMQGR LRQLLVGKTA VSGPKGLGRG EITAEKLEEI APGLWWQIAL
DDEKAMGELE ALRKQFDDAR KRLDRRFEDK VDKLQRGDEL PPGVMKMVKV FVAVKRKLQP
GDKMAGRHGN KGVISKILPI EDMPYLEDGT SVDIVLNPLG VPSRMNVGQI FETHLGWAAA
GLGKQVQRLL EDWQHGGQKQ ALIEHLRDVY GPDEELPDTE EELVELARNL SKGIPFATPV
FDGAHIDDIE NLLEKAGLDR SGQSYLYDGQ SGERFKRPVT VGYIYMLKLH HLVDDKIHAR
SIGPYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT LQEMLTVKSD DVAGRTKVYE
SIVRGDDTFE AGIPESFNVL VKEMRSLGLN VELENS
