ID   RPOB_PHYPA              Reviewed;        1085 AA.
AC   P60283;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta;
OC   Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrium.
OX   NCBI_TaxID=3218;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Gransden 2004;
RX   PubMed=12954768; DOI=10.1093/nar/gkg726;
RA   Sugiura C., Kobayashi Y., Setsuyuki A., Sugita C., Sugita M.;
RT   "Complete chloroplast DNA sequence of the moss Physcomitrella patens:
RT   evidence for the loss and relocation of rpoA from the chloroplast to the
RT   nucleus.";
RL   Nucleic Acids Res. 31:5324-5331(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AP005672; BAC85073.1; -; Genomic_DNA.
DR   RefSeq; NP_904223.1; NC_005087.1.
DR   AlphaFoldDB; P60283; -.
DR   SMR; P60283; -.
DR   PRIDE; P60283; -.
DR   GeneID; 2546814; -.
DR   KEGG; ppp:2546814; -.
DR   InParanoid; P60283; -.
DR   OrthoDB; 944344at2759; -.
DR   Proteomes; UP000006727; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1085
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048039"
SQ   SEQUENCE   1085 AA;  124109 MW;  6794ACA24FA83F8F CRC64;
     MKKIITLSAP PPSQFSFLSE FQFSLPELRQ IQFKSYYYFI YKNLISELNI FPEIFDLNQE
     FQFELLNKEY KLIKPEKTTI KFHYNTYSSD LYVTCRLLRR KKKIEIQKQT IFIGSIPLID
     YQSTFRINSV TRVIINQILR SPGIYYNSEL DHNGISIYTG TIISDWGGRL KLEIDSKTRI
     WARISKKRKV SILVLLLAMG LTIKQILDSV CSSKIFLDFL KEKKKKKEHL QSTEDAMVEL
     YKQLYYIGGD LLFSESIRKE LQKKFFQQRC ELGKIGRLNV NKKLSLDIPE NEFFLLPQDI
     LAAIDYLIKI KFGIGTLDDI DHLKNRRIRS VADLLQDQLK LALIRLENSV RQVMRRTTKR
     KRLLSPKNLI TQTPLIATFK EFFGSHPLSQ FLDQTNSLAE IVHKRRLSSL GPGGVTRRTA
     GFQVRDIHFS HYTRICPIET SEGMNAGLIA SLAIHANVNN WGFLESPFYK ISKNVKEEKI
     INLSAGEDEY YRIATGNCLA LDQGTQKIQI TPARYRQEFL AIAWEQIHLR SIYPLQYFSV
     GVSLIPFLEH NDANRALMGS NMQRQAVPLI KLEKCIVGTG LESQVALDSG NVMITKQSEK
     IMYTDGKKIS LLNNTNETVN THLIIYQRSN NSTCIHQKPQ VISKKFLKKG QVLTDGAAIL
     KGELTLGKNI LVAYMPWEGY NFEDAILISE RLIYEDIYTS IHIERYEIES RNTNQGPEKI
     TKEIPHLENS VLRHLDKNGL VIPGSWVETG DVLVGKLTPQ ETEESLRAPE GKLLQAIFGI
     QVTNAKETCL KVPLNGKGRV IDVIWISKKE NSSNYEKIIH VYIAQKRKIQ VGDKVAGRHG
     NKGIISKILP RQDMPYLQDG TPIDMVLSPL GVPSRMNVGQ IFECLLGLAG YFLGKHYRIT
     PFDEKYEREA SRKLVFSELY KASKKTGNLW LFEPENPGKS RLLNGRTGEI FEQAVTVGKA
     YMLKLIHQVD DKIHARSSGP YALVTQQPLR GRSRRGGQRV GEMEVWALEG FGVAYILQEM
     LTIKSDHIHA RYEVLGAIIT GEPIPKPKTA PESFLLLVRE LRSLSLELDH AVIFEKNLNI
     KFKDV