ID   RPOB_PLAF7              Reviewed;        1024 AA.
AC   P21421;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta;
DE            Short=RNA polymerase subunit beta;
GN   Name=rpoB;
OS   Plasmodium falciparum (isolate 3D7).
OG   Plastid; Apicoplast.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX   NCBI_TaxID=36329;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BW/C10;
RX   PubMed=7808472; DOI=10.1016/0166-6851(94)90149-x;
RA   Gardner M.J., Goldman N., Barnett P., Moore P.W., Rangachari K., Strath M.,
RA   Whyte A., Williamson D.H., Wilson R.J.M.;
RT   "Phylogenetic analysis of the rpoB gene from the plastid-like DNA of
RT   Plasmodium falciparum.";
RL   Mol. Biochem. Parasitol. 66:221-231(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BW/C10;
RX   PubMed=8757284; DOI=10.1006/jmbi.1996.0449;
RA   Wilson R.J.M., Denny P.W., Preiser P.R., Rangachari K., Roberts K., Roy A.,
RA   Whyte A., Strath M., Moore D.J., Moore P.W., Williamson D.H.;
RT   "Complete gene map of the plastid-like DNA of the malaria parasite
RT   Plasmodium falciparum.";
RL   J. Mol. Biol. 261:155-172(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 328-1024.
RX   PubMed=2011147; DOI=10.1016/0166-6851(91)90227-w;
RA   Gardner M.J., Williamson D.H., Wilson R.J.M.;
RT   "A circular DNA in malaria parasites encodes an RNA polymerase like that of
RT   prokaryotes and chloroplasts.";
RL   Mol. Biochem. Parasitol. 44:115-124(1991).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (Potential).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Plastid, apicoplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; X75544; CAA53232.1; -; Genomic_DNA.
DR   EMBL; X95275; CAA64572.1; -; Genomic_DNA.
DR   EMBL; X52177; CAA36427.1; -; Genomic_DNA.
DR   PIR; S72282; RNZQBF.
DR   AlphaFoldDB; P21421; -.
DR   SMR; P21421; -.
DR   STRING; 36329.P21421; -.
DR   PRIDE; P21421; -.
DR   VEuPathDB; PlasmoDB:PF3D7_API04400; -.
DR   InParanoid; P21421; -.
DR   PhylomeDB; P21421; -.
DR   Proteomes; UP000001450; Apicoplast A.
DR   GO; GO:0020011; C:apicoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Apicoplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1024
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048070"
SQ   SEQUENCE   1024 AA;  122186 MW;  B49FBDED7C9BD412 CRC64;
     MIYIVNPILV KNNYIISNLY LLLIQEIIYN LRYYILFLNN NINVKFNFIY YKIIILLTNI
     NINSIDTIQN INNLLKIILT LKLNFININK IIKFNILIFI LPFIYNNIII LNGLYKTCIQ
     LFKKNNKIFI IKFKNNNKNI IYVYIYISLG LRIIFKISKL NIDCYFNNFK FNFLILLLYL
     NNIYINKNIS LFIYNNIINK KILIYNYIKF IYSKYNNINN IISLKLFIIK LNKFNNIYIN
     LLNILFSIKL NFSYYSDFYI NNIYNKKFYS IIDNLLIKSK KYLKIFKYQL LNINRNIYNN
     ITLLLNNKKY INIILENINI NPLVQYSDQV NNLSEINQKF KINMITTGLN SKFILNNDLR
     ELPRNILGYI SLINTNEGLT CGLVNYLTTN IFLNLKYLFV IYYKHIFYNR YNFKLLLNIF
     NKNFYNISFN NIYLKKNINF NKTTILTINK NTFKICNITQ NIIYIPFNYL LSFIENLIPF
     IHYNDSIRNL MSIKMHTQIV PIIYPNLSNI ITNYNFILNK YLNHLIISYQ EGIVIYVSCI
     KIIIRDLFNR QIIYYLNNYK KINQNILLIY KPIVWVGEKV NIGQILAINS NLLNSEYSLG
     NNLLVGYGSY LGYEYEDAII ISRKILYNNL YTSLHLNIYE ISLNIINNIP EICSINLSKM
     YYKNIKHLDK YGIIKEGTYI LANNILISKL MFMPFIFNNK SLINIINFLF GSKLRIFKNK
     PIISTIHDIG RVIKIEILPN HLYNKTEKNN IYLKFRIYIG IQKYLQLGDK ICNRHGHKGI
     ISYISEINDI PYLNNKIQPD IFISAISIPS RINIGQIFEG IYGLNSLYLN TRYIISNNLN
     KNYYNNYNHI FNYYKYNYNN NFNINSKMSY NYNKYYLKNP FTGNMINNSI CLNNIYYYKL
     IHMIKDKFRY RFIGLYSELT QQPIKGNTKQ GGQRFGEMEV WALEAFGASY LFKEFFTYKS
     DDIKSRKILK NYLFNNYKIK NTFISETFKL ILKELQSLAI NIEAFCIFND TNNLLENLPI
     NIIY