ID   RPOB_PLAOC              Reviewed;        1070 AA.
AC   Q09G54;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Platanus occidentalis (Sycamore) (American plane tree).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Proteales; Platanaceae; Platanus.
OX   NCBI_TaxID=4403;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16934154; DOI=10.1186/1471-2229-6-17;
RA   Moore M.J., Dhingra A., Soltis P.S., Shaw R., Farmerie W.G., Folta K.M.,
RA   Soltis D.E.;
RT   "Rapid and accurate pyrosequencing of angiosperm plastid genomes.";
RL   BMC Plant Biol. 6:17-17(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; DQ923116; ABI49770.1; -; Genomic_DNA.
DR   RefSeq; YP_740557.1; NC_008335.1.
DR   AlphaFoldDB; Q09G54; -.
DR   SMR; Q09G54; -.
DR   GeneID; 4271289; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300452"
SQ   SEQUENCE   1070 AA;  120663 MW;  8F5B73B0FCB33BA6 CRC64;
     MRRDGNEGMS TIPGFNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLIKERDAV YESLTYSSEV YVPAGLIWKP GRDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSESDH NGISVYTGTI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
     LVPSSAMGSN LREILDNVCY PEIFLSFLND KEKKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNRRL NLDIPQNNTF LLPRDVLAAA DHLIGMKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALI RLENVVRGTI CGAIKHKLIP TPQNLVTSTP
     LTTTYESFFG LHPLSQVLDR TNPLTQMVHG RKLSYLGPGG LTGRTASFRI RDIHSSHYGR
     ICPIDTSEGI NVGLIGSLAI YARIGHWGSL ESPFYEISDR SKEVQMLYLS PSRDEYYMVA
     AGNSLALNQS IPEEQVVPAR YRQEFLTIAW EQVHFRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSQSEK CIVGTGLERQ AALDSGVSTI AEHEGKIIYT DTDKIILLGN
     GDTLSIPLVM YQRSNKNTCM YQKPQVRRGK FIKKGQIVAD GAATVGGELA LGKNVLVAYM
     PWEGYNSEDA VLISERLVYG DIYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL
     DRNGIVMLGS WVETGDILVG KLTPQMAKES SYAPEDRLLR AILGIQVSTS KETCLKLPIG
     GRGRVIDVRW IQKKGGSSYN PETICVYISQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
     YLQDGTPIDM VFNPLGVPSR MNVGQIFECS LGLAGDLLDR HYRIAPFDER YEQEASRKLV
     FSELYKASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEQPV LIGKSYILKL IHQVDDKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIRARQEVL
     GTTIVGGTIP SPEDAPESFR LLVRELRSLA LELNHFLVSE KNFQINRKEA