ID   RPOB_POLAQ              Reviewed;        1366 AA.
AC   A4SUV4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Pnuc_0046;
OS   Polynucleobacter asymbioticus (strain DSM 18221 / CIP 109841 /
OS   QLW-P1DMWA-1) (Polynucleobacter necessarius subsp. asymbioticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=312153;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18221 / CIP 109841 / QLW-P1DMWA-1;
RX   PubMed=22675600; DOI=10.4056/sigs.2395367;
RA   Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G.,
RA   Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M.,
RA   Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.;
RT   "Complete genome sequence of Polynucleobacter necessarius subsp.
RT   asymbioticus type strain (QLW-P1DMWA-1(T)).";
RL   Stand. Genomic Sci. 6:74-83(2012).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000655; ABP33268.1; -; Genomic_DNA.
DR   RefSeq; WP_011901894.1; NC_009379.1.
DR   AlphaFoldDB; A4SUV4; -.
DR   SMR; A4SUV4; -.
DR   STRING; 312153.Pnuc_0046; -.
DR   PRIDE; A4SUV4; -.
DR   EnsemblBacteria; ABP33268; ABP33268; Pnuc_0046.
DR   GeneID; 31480392; -.
DR   KEGG; pnu:Pnuc_0046; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_4; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000000231; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1366
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000086376"
SQ   SEQUENCE   1366 AA;  152502 MW;  9912102954DB396B CRC64;
     MNYSFTERKR VRKSFAKRVN NHQVPYLIAT QLESYAKFLQ ADKPAMSRLT EGLQAAFTSA
     FPIVSNNGYA RMEYVSYQLS QPPFDVKECQ QRGYTYHSAL RAKVRLIIYD REAPTKVKEV
     KESEVYMGEI PLMTENGSFV INGTERVIVS QLHRSPGVFF EHDKGKTHSS GKLLFSARII
     PYRGSWLDFE FDPKDILYFR VDRRRKMPVT ILLKAIGLNN EQILANFFNF DHFSLTANGG
     SMEFVPERLR GQLANFDVLD KNGVVVIQKD KRINAKHIRE LEAAKTKTIA VPDDYLVGRV
     VARNIVDPDS GEILAYANDE ITEELLATLR DAGIKQLETI YTNDLDSGAY ISQTLRTDET
     ADQMAARIAI YRMMRPGEPP TEDAVEALFQ RLFYNEDTYD LSRVGRMKVN SRLNRPEMEG
     PMVLSNEDIL DTIKSLVDLR NGKGEVDDID HLGNRRVRCV GELAENQFRA GLSRVERAVK
     ERLGQAETEN LMPHDLINSK PISSAIREFF GSSQLSQFMD QTNPLSEITH KRRISALGPG
     GLTRERAGFE VRDVHPTHYG RVCPIETPEG PNIGLINSLA LFARLNEHGF LETPYRKVSN
     SKVSDEVVYL SAIEEAKYVI AQANATIDKS GKLADELVSA RQAGETIMVS PERIDFIDVA
     PSQIVSAAAS LVPFLEHDDA NRALMGANMS RQAVPCLRPD KPLVGTGLER IVAVDSGTVI
     LATRGGIVDY VDANRIVIRV NDDETAAGEV GVDIYNLIKY TRSNQNTNIN QRPIVQAGDR
     VVRGDVVADG ASTDLGELAL GQNMTVAFMP WNGYNFEDSI LISEKVVAED RYTSIHIEEL
     SVVARDTKLG SEEITRDISN LAESQLSRLD ESGIVYIGAE VEAGDVLVGK VTPKGETTLT
     PEEKLLRAIF GEKASDVKDT SLRVPSGMIG TVIDVQVFTR EGIERDARAQ AIIQEELQRY
     RLDLNDQLRI VEGDAFMRLE KLLIGKVANG GPQKLAKGTK IDKEYLASLD KYHWFDVRPA
     DEEVATQVEA IKSSIEAKRK QFDEAFEEKR TKLTQGDDLQ AGVTKMVKVY LAVKRRLQPG
     DKMAGRHGNK GVVSKIAPAE DMPFMADGRP VDIVLNPLGV PSRMNVGQIL ETHLGWAAQG
     IGKRIDEMVR QQVKQAELRK FLKQLYNETG RIEDIDNFTD EQITVLAENL RQGLPFATPV
     FDGATEAEIG RMLELAYPEE VATSLKMTPS RQQMILCDGR TGDQFERPVT VGVMHVLKLH
     HLVDDKMHAR STGPYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGASYV LQEMLTVKSD
     DVAGRTKVYE NIVKGEHTID AGMPESFNVL VKEIRSLGID IDMERN