RPOB_POLNA
ID RPOB_POLNA Reviewed; 1370 AA.
AC A1VTF8;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Pnap_3640;
OS Polaromonas naphthalenivorans (strain CJ2).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Polaromonas.
OX NCBI_TaxID=365044;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CJ2;
RX PubMed=19453698; DOI=10.1111/j.1462-2920.2009.01947.x;
RA Yagi J.M., Sims D., Brettin T., Bruce D., Madsen E.L.;
RT "The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal
RT tar-contaminated sediment, reveals physiological and metabolic versatility
RT and evolution through extensive horizontal gene transfer.";
RL Environ. Microbiol. 11:2253-2270(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000529; ABM38936.1; -; Genomic_DNA.
DR RefSeq; WP_011803003.1; NC_008781.1.
DR AlphaFoldDB; A1VTF8; -.
DR SMR; A1VTF8; -.
DR STRING; 365044.Pnap_3640; -.
DR PRIDE; A1VTF8; -.
DR EnsemblBacteria; ABM38936; ABM38936; Pnap_3640.
DR KEGG; pna:Pnap_3640; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000644; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1370
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300367"
SQ SEQUENCE 1370 AA; 152543 MW; CF1F6132AE8A105A CRC64;
MAYSFTERKR IRKSFGNRES VLTVPYLLQM QKDAYIAFLQ ADRAPQKRMV EGLQAAFQNA
FPIVSHNGFV EMKFIEYNLA KPAFDVRECQ TRGLTFASAV RAKVQLIIYD RESSTSQSKV
VKEVKEQEVY MGEVPLMTDK GSFVINGTER VIVSQLHRSP GVFFEHDKGK THSSGKLLFS
ARIIPYRGSW LDFEFDPKDI LYFRVDRRRK MPVTILLKAI GLNHESILAN FFVFDNFRLM
DSGAQMEFVA ERMRGEVARF DLTDKAGKVV VAKDKRITVR HTRELEQSGT TSISVPEDFL
IGRVVAKNII DTDTGEIIAK ANDELTELLL KKLRTAGIQN LQVLYTNELD QGAYISQTLR
ADETADEFAA RVAIYRMMRP GEPPTEDAVQ ALFQRLFYNP DTYDLSRVGR MKFNARVGRD
ESTGPMVMTN EDILAVVKIL VDLRNGNGEV DDIDHLGNRR VRCVGELAEN QYRTGLARIE
KAVKERLGQA EQEPLMPHDL INSKPISAAL KEFFGASQLS QFMDQTNPLA EITHKRRVSA
LGPGGLTRER AGFEVRDVHV THYGRVCPIE TPEGPNIGLI NSLALYARLN EYGFIETPYR
RVADSKVTME IDYLSAIEEG KYVIAQANAA LDNEGRLTGD LVSAREKGES ILVSAERVQY
MDVSPAQIVS VAASLVPFLE HDDANRALMG ANMSRQAVPV LRPEKPLVGT GIERVAAVDS
GTVVTATRGG VVDYVDATRI VVRVNDAEAL AGEIGVDIYN LIKYQRSNQN TNIHQRPIVK
MGDKLAKGDV VADGASTDLG EIAIGQNMLI GFMPWNGYNF EDSILISERV VAEDRYTSIH
IEELVVMARD TKLGSEEITR DIPNLSEQQL NRLDESGIIY VGAEVQPGDT LVGKVTPKGE
TTLTPEEKLL RAIFGEKASD VKDTSLRVSQ GSSGTVIDVQ VFTREGITRD KRAQQIIDDE
LKRYRLDLND QLRIVEADAF DRIEKLLIGK IANGGPKKLA KGTKLDKAYL TDVEKYHWFD
IRPADDEVAS QLESIKNSME QTRHSFDLSF EEKRKKLTQG DELPSGVLKM VKVYLAVKRR
LQPGDKMAGR HGNKGVVSKI VPVEDMPHMA DGTPCDIVLN PLGVPSRMNV GQVLEVHLGW
AAKGLGQRIG DMLQAESKVA ELRQFMDTLY NKSGRTEDLA SLSDEQVVEM AQNLTTGVPF
ATPVFDGASE ADIMTMLQLA YPDAVAKAKG LTPTRTQAQL YDGRTGDAFE RTTTVGYMHF
LKLHHLVDDK MHARSTGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYVLQEMLT
VKSDDVVGRT KVYESIVKGE HAITAGMPES FNVLVKEIRS LGIDMELERS
