ID   RPOB_POPAL              Reviewed;        1070 AA.
AC   Q14FG5;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Populus alba (White poplar).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=43335;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Okumura S., Yamashita A., Kanamoto H., Hattori M., Takase H., Tomizawa K.;
RT   "Complete structure of the chloroplast genome of Populus alba.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AP008956; BAE97197.1; -; Genomic_DNA.
DR   RefSeq; YP_665550.1; NC_008235.1.
DR   AlphaFoldDB; Q14FG5; -.
DR   SMR; Q14FG5; -.
DR   GeneID; 4178254; -.
DR   KEGG; palz:4178254; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276596"
SQ   SEQUENCE   1070 AA;  120521 MW;  16F79B3FA3C3E548 CRC64;
     MLGDGNGGMS TIPGFNQIQF EGFCRFIDQG LAEELYKFPK IEDRDQEIEF QLFVETYQLV
     EPSIKERDAV YESLTYSSEL YVSGGLIWKN SRDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELNH NGISVYTGTI ISDWGGRVEL EIDKKARIWA RVSRKQKISI
     LVLSSAMGLN LREILENVCY PEIFLSFLSD KEKKKIGSRE NAILEFYQQF TCVGGGPVFS
     ESLCKELQKK FFQQRCELGR IGRLNMNQRL NLDIPHNNTF LLPRDILAAA DHLIGMKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALI RLENVVRGTI CGAIRHKLIP TPQNLVTSTP
     LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKSSYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLTI HAKIGHLGSL ESPFYEISAR SKKVRMLYLS PNRDEYYMIA
     AGNCLALNRG AREEQVVPAR YRQEFLTIAW EQVRLRSFFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLARSEK CIVGTGLERQ VALDSGVPAI AEHEGKIIYT DIDKIILSGN
     GYTVSIPLVM YQRSNKNTCM HQKTQVQRGK CIKRGQVLAD GAATVGGELA LGKNILVAYM
     PWEGYNFEDA VLISERLVYE DVYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL
     DKNGIVMLGS WVETGDILIG KLTPQLAKES SYAPEDRLLR AILGIQVSTS KETCLKLPTG
     GRGRVIDVRW IQKKGGSSYN PETIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
     YLQDGGPVDM VFNPLGVPSR MNVGQIFECS LGLAGSLLAR HYRVAPFDER YEQEASRKLV
     FSELYEAGKQ TANPWVFEPE CPGKSRIFDG RTGDPFEQPV IIGKPYILKL IHQVADKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVSH ILQEMLTYKS DHIRARQEVL
     GTTISGRTIP KPEDAPESFR LLVRELRSLA LELKHFLISE KNFQINRKEV